Division of Crop Improvement, ICAR-Indian Institute of Pulses Research, Kanpur, India.
Department of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
Heredity (Edinb). 2022 Jun;128(6):473-496. doi: 10.1038/s41437-022-00513-5. Epub 2022 Mar 5.
In the last decade, advancements in genomics tools and techniques have led to the discovery of many genes. Most of these genes still need to be characterized for their associated function and therefore, such genes remain underutilized for breeding the next generation of improved crop varieties. The recent developments in different reverse genetic approaches have made it possible to identify the function of genes controlling nutritional, biochemical, and metabolic traits imparting drought, heat, cold, salinity tolerance as well as diseases and insect-pests. This article focuses on reviewing the current status and prospects of using reverse genetic approaches to breed nutrient-rich and climate resilient cereal and food legume crops.
在过去的十年中，基因组学工具和技术的进步导致了许多基因的发现。这些基因中的大多数仍需要对其相关功能进行特征描述，因此，这些基因在培育下一代改良作物品种方面的应用仍然不足。不同反向遗传学方法的最新进展使得鉴定控制营养、生化和代谢特性的基因的功能成为可能，这些特性赋予了作物耐旱、耐热、耐寒、耐盐以及抗病虫的能力。本文重点回顾了利用反向遗传学方法培育营养丰富和适应气候变化的谷类和豆科作物的现状和前景。
