Mallinson David, Cheung David L, Simionesie Dorin, Mullen Alexander B, Zhang Zhenyu J, Lamprou Dimitrios A
Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, 161 Cathedral Street, Glasgow, United Kingdom.
School of Chemistry, National University of Ireland, Galway, University Road, Galway, Ireland.
J Biomed Mater Res A. 2017 Mar;105(3):737-745. doi: 10.1002/jbm.a.35949. Epub 2016 Nov 7.
Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. Anastellin and superfibronectin have been seen to exhibit antiangiogenic properties and other properties that may make it suitable for consideration for incorporation into biomaterials. The molecular interaction was directly quantified by atomic force microscope (AFM)-based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). Using AFM, it was found that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN nm ) than on poly (methyl methacrylate) films (68.6 pN nm ). However, this is not consistent with the QCM adsorption measurements, which show no significant difference. Molecular dynamics simulations of the behavior of anastellin on polyurethane in aqueous solution were performed to rationalize the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 737-745, 2017.
通过结合实验和计算方法，研究了纤连蛋白的重组片段阿纳斯肽与代表性生物材料表面之间的相互作用。阿纳斯肽和超纤连蛋白已被证明具有抗血管生成特性以及其他可能使其适合考虑纳入生物材料的特性。分子相互作用通过基于原子力显微镜（AFM）的力谱直接定量，并辅以使用石英晶体微天平（QCM）的吸附测量。使用AFM发现，阿纳斯肽分子在聚氨酯薄膜上的粘附力（72.0 pN nm）比在聚甲基丙烯酸甲酯薄膜上的粘附力（68.6 pN nm）更强。然而，这与QCM吸附测量结果不一致，QCM吸附测量结果显示没有显著差异。进行了阿纳斯肽在水溶液中在聚氨酯上行为的分子动力学模拟，以合理解释实验数据，并表明阿纳斯肽能够快速吸附到PU上，而其二级结构在水中吸附时保持稳定。© 2016威利期刊公司。《生物医学材料研究杂志》A部分：105A：737 - 745，2017年。
