Department of Industrial Chemistry, Faculty of Engineering, Tokyo University of Science, 12-1 Ichigayafunagawara-machi, Shinjuku-ku, Tokyo 162-0826, Japan.
Department of Applied Chemistry, Kyushu University, 744 Nishi-ku, Motooka, Fukuoka 819-0395, Japan.
Colloids Surf B Biointerfaces. 2015 Jun 1;130:77-83. doi: 10.1016/j.colsurfb.2015.04.010. Epub 2015 Apr 10.
We recently developed a system for coating polystyrene (PS) substrates with hydroxyapatite (HAp) by utilizing serum protein adsorption layers as mediators to induce the heterogeneous nucleation of HAp in simulated body fluids (SBFs). In this study, the selective deposition of HAp on polymer substrate surfaces with different protein adsorption abilities was investigated using PS and poly(methyl methacrylate) (PMMA). Atomic force microscopic observations and the results of a quantitative analysis using a quartz-crystal microbalance (QCM) revealed that the amounts of proteins such as human serum albumin (HSA) and human immunoglobulin G (hIgG) adsorbed on PS substrate surfaces were markedly greater than those on PMMA substrate surfaces. A markedly larger amount of HAp was deposited on protein-treated PS substrate surfaces than on PMMA substrate surfaces, reflecting protein adsorption to polymers. We also revealed that the deposition of HAp on protein-adsorbed PS substrate surfaces was enhanced by aqueous calcium chloride treatments before immersion in 1.5SBF. In the case of 2.5 M calcium chloride treatment, these surfaces were completely covered with deposits.
我们最近开发了一种在聚苯乙烯 (PS) 基底上涂覆羟基磷灰石 (HAp) 的系统，利用血清蛋白吸附层作为介导物来诱导 HAp 在模拟体液 (SBF) 中的异质成核。在这项研究中，我们使用 PS 和聚甲基丙烯酸甲酯 (PMMA) 研究了具有不同蛋白质吸附能力的聚合物基底表面上 HAp 的选择性沉积。原子力显微镜观察和石英晶体微天平 (QCM) 的定量分析结果表明，人血清白蛋白 (HSA) 和人免疫球蛋白 G (hIgG) 等蛋白质在 PS 基底表面上的吸附量明显大于在 PMMA 基底表面上的吸附量。在蛋白质处理的 PS 基底表面上沉积的 HAp 明显多于 PMMA 基底表面上的沉积量，这反映了蛋白质对聚合物的吸附。我们还揭示了在浸入 1.5SBF 之前用氯化钙水溶液处理可以增强蛋白质吸附的 PS 基底表面上 HAp 的沉积。在 2.5M 氯化钙处理的情况下，这些表面完全被沉积物覆盖。
