磷脂酶C-β1与细胞分化：对肌发生和成骨作用的深入了解

PLC-β1 and cell differentiation: An insight into myogenesis and osteogenesis.

作者信息

Ramazzotti Giulia, Faenza Irene, Fiume Roberta, Billi Anna Maria, Manzoli Lucia, Mongiorgi Sara, Ratti Stefano, McCubrey James A, Suh Pann-Ghill, Cocco Lucio, Follo Matilde Y

机构信息

Cellular Signalling Laboratory, Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.

出版信息

Adv Biol Regul. 2017 Jan;63:1-5. doi: 10.1016/j.jbior.2016.10.005. Epub 2016 Oct 18.

DOI:10.1016/j.jbior.2016.10.005

PMID:27776973

Abstract

Phosphoinositide-phospholipase C-β1 (PLC-β1) plays a crucial role in the initiation of the genetic program responsible for muscle differentiation and osteogenesis. During myogenic differentiation of murine C2C12 myoblasts, PLC-β1 signaling pathway involves the Inositol Polyphosphate Multikinase (IPMK) and β-catenin as downstream effectors. By means of c-jun binding to cyclin D3 promoter, the activation of PLC-β1 pathway determines cyclin D3 accumulation. However, osteogenesis requires PLC-β1 expression and up-regulation but it does not affect cyclin D3 levels, suggesting that the two processes require the activation of different mediators.

摘要

磷酸肌醇磷脂酶C-β1（PLC-β1）在启动负责肌肉分化和成骨的遗传程序中起着关键作用。在小鼠C2C12成肌细胞的肌源性分化过程中，PLC-β1信号通路涉及肌醇多磷酸多激酶（IPMK）和β-连环蛋白作为下游效应器。通过c-jun与细胞周期蛋白D3启动子结合，PLC-β1通路的激活决定了细胞周期蛋白D3的积累。然而，成骨需要PLC-β1的表达和上调，但它不影响细胞周期蛋白D3的水平，这表明这两个过程需要激活不同的介质。