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利用AMMI分析和GGE双标图对不同环境下用于乙醇生产的选定甜高粱基因型进行适应性和稳定性研究。

Adaptability and Stability Study of Selected Sweet Sorghum Genotypes for Ethanol Production under Different Environments Using AMMI Analysis and GGE Biplots.

作者信息

Rono Justice Kipkorir, Cheruiyot Erick Kimutai, Othira Jacktone Odongo, Njuguna Virginia Wanjiku, Macharia Joseph Kinyoro, Owuoche James, Oyier Moses, Kange Alex Machio

机构信息

Department of Biochemistry and Molecular Biology, Egerton University, P.O. Box 536-20115, Egerton, Kenya.

Department of Crops, Horticulture and Soil, Egerton University, P.O. Box 536-20115, Egerton, Kenya.

出版信息

ScientificWorldJournal. 2016;2016:4060857. doi: 10.1155/2016/4060857. Epub 2016 Sep 29.

Abstract

The genotype and environment interaction influences the selection criteria of sorghum () genotypes. Eight sweet sorghum genotypes were evaluated at five different locations in two growing seasons of 2014. The aim was to determine the interaction between genotype and environment on cane, juice, and ethanol yield and to identify best genotypes for bioethanol production in Kenya. The experiments were conducted in a randomized complete block design replicated three times. Sorghum canes were harvested at hard dough stage of grain development and passed through rollers to obtain juice that was then fermented to obtain ethanol. Cane, juice, and ethanol yield was analyzed using the additive main effect and multiplication interaction model (AMMI) and genotype plus genotype by environment (GGE) biplot. The combined analysis of variance of cane and juice yield of sorghum genotypes showed that sweet sorghum genotypes were significantly ( < 0.05) affected by environments (E), genotypes (G) and genotype by environment interaction (GEI). GGE biplot showed high yielding genotypes EUSS10, ACFC003/12, SS14, and EUSS11 for cane yield; EUSS10, EUSS11, and SS14 for juice yield; and EUSS10, SS04, SS14, and ACFC003/12 for ethanol yield. Genotype SS14 showed high general adaptability for cane, juice, and ethanol yield.

摘要

基因型与环境的相互作用影响着高粱基因型的选择标准。在2014年的两个生长季节中,于五个不同地点对八个甜高粱基因型进行了评估。目的是确定基因型与环境在茎秆、汁液和乙醇产量方面的相互作用,并确定肯尼亚用于生物乙醇生产的最佳基因型。试验采用随机完全区组设计,重复三次。在谷物发育的硬面团阶段收获高粱茎秆,通过滚筒压榨获得汁液,然后将汁液发酵以获取乙醇。使用加性主效应和乘法交互作用模型(AMMI)以及基因型加基因型与环境互作(GGE)双标图对茎秆、汁液和乙醇产量进行分析。高粱基因型茎秆和汁液产量的联合方差分析表明,甜高粱基因型受环境(E)、基因型(G)以及基因型与环境互作(GEI)的显著影响(P < 0.05)。GGE双标图显示,茎秆产量方面的高产基因型为EUSS10、ACFC003/12、SS14和EUSS11;汁液产量方面为EUSS10、EUSS11和SS14;乙醇产量方面为EUSS10、SS04、SS14和ACFC003/12。基因型SS14在茎秆、汁液和乙醇产量方面表现出较高的一般适应性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb49/5061992/817797a03e49/TSWJ2016-4060857.001.jpg

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