Alshatwi Ali A, Athinarayanan Jegan, Periasamy Vaiyapuri Subbarayan, Alatiah Khalid A
Nanobiotechnology and Molecular Biology Research Laboratory, Department of Food Science and Nutrition, College of Food Science and Agriculture, King Saud University, P.O. Box 2460, Riyadh, 11451, Kingdom of Saudi Arabia.
Appl Biochem Biotechnol. 2017 Feb;181(2):725-734. doi: 10.1007/s12010-016-2244-z. Epub 2016 Oct 24.
Nanographene- and graphene-based nanohybrids have garnered attention in the biomedical community owing to their biocompatibility, excellent aqueous processability, ease of cellular uptake, facile surface functionalization, and thermal and electrical conductivities. NiO nanoparticle-graphene nanohybrid (G-NiO) was synthesized by first depositing Ni(OH) onto the surface of graphene oxide (GO) sheets. The Ni(OH)-GO hybrids were then reduced to G-NiO using date palm syrup at 85 °C. The prepared G-NiO nanohybrids were characterized by X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy, and energy-dispersive X-ray spectroscopy (EDX). The NiO nanoparticles, with a diameter of approximately 20-30 nm, were uniformly dispersed over the surface of the graphene sheets. The G-NiO hybrids exhibit biocompatibility in human mesenchymal stem cells (hMSCs) up to 100 μg/mL. The nanohybrids do not cause any significant changes in cellular and nuclear morphologies in hMSCs. The as-synthesized nanohybrids show excellent biocompatibility and could be a promising material for biomedical applications.
基于纳米石墨烯和石墨烯的纳米杂化物因其生物相容性、出色的水相加工性、易于细胞摄取、表面功能化简便以及热导率和电导率而在生物医学领域受到关注。通过首先将Ni(OH)沉积在氧化石墨烯(GO)片的表面上，合成了NiO纳米颗粒-石墨烯纳米杂化物(G-NiO)。然后使用枣椰糖浆在85°C下将Ni(OH)-GO杂化物还原为G-NiO。通过X射线衍射(XRD)、场发射扫描电子显微镜(FESEM)、傅里叶变换红外光谱和能量色散X射线光谱(EDX)对制备的G-NiO纳米杂化物进行了表征。直径约为20-30nm的NiO纳米颗粒均匀地分散在石墨烯片的表面上。G-NiO杂化物在高达100μg/mL的人间充质干细胞(hMSCs)中表现出生物相容性。纳米杂化物不会在hMSCs的细胞和核形态上引起任何显著变化。合成的纳米杂化物显示出优异的生物相容性，可能是生物医学应用的有前途的材料。
