Chehroudi B, Gould T R, Brunette D M
Department of Oral Biology, Faculty of Dentistry, University of British Columbia, Vancouver, Canada.
J Biomed Mater Res. 1989 Sep;23(9):1067-85. doi: 10.1002/jbm.820230907.
The effects of a grooved titanium-coated substratum on epithelial (E) cell behavior were studied in vitro and in vivo. V-shaped grooves, 10 microns deep, were produced in silicon wafers by micromachining, a process which was developed for the fabrication of microelectronic components. The grooved substrata were replicated in epoxy resin and coated with 50 nm of titanium. More E cells were found attached to the grooved titanium surfaces than to adjacent smooth surfaces. In comparison to the smooth surfaces where clusters of E cells were randomly oriented, on the grooved surfaces, clusters of E cells were markedly oriented along the long axis of grooves. Grooved and smooth titanium-coated epoxy implants were placed percutaneously in the parietal area of rats. Electron and light microscopic observations indicated that E cells were tightly attached to the implant surfaces and this attachment is through basal lamina-like and hemidesmosome-like structures. In the grooved portion of the implant, E cells interdigitated into the grooves and had rounded nuclei. Histomorphometric measurements indicated that there was a shorter length of epithelial attachment, longer length of connective tissue attachment, and less recession in the grooved, compared to the smooth portion of implants after 7 and 10 days. These results indicate that horizontal grooves produced by micromachining can significantly impede epithelial downgrowth on titanium-coated epoxy implants.
研究了带凹槽的钛涂层基质对上皮（E）细胞行为的体内外影响。通过微加工在硅片上制作出深度为10微米的V形凹槽，该工艺是为制造微电子元件而开发的。将带凹槽的基质复制到环氧树脂中，并用50纳米的钛进行涂层。发现附着在带凹槽钛表面的E细胞比相邻光滑表面上的更多。与E细胞簇随机取向的光滑表面相比，在带凹槽表面，E细胞簇明显沿凹槽长轴取向。将带凹槽和光滑的钛涂层环氧树脂植入物经皮放置在大鼠的顶叶区域。电子显微镜和光学显微镜观察表明，E细胞紧密附着在植入物表面，这种附着是通过基膜样和半桥粒样结构实现的。在植入物的带凹槽部分，E细胞相互交错进入凹槽，细胞核呈圆形。组织形态计量学测量表明，与植入物光滑部分相比，7天和10天后，带凹槽部分上皮附着长度较短，结缔组织附着长度较长，退缩较少。这些结果表明，微加工产生的水平凹槽可显著阻碍钛涂层环氧树脂植入物上的上皮向下生长。
