Chehroudi B, Soorany E, Black N, Weston L, Brunette D M
Department of Oral Biology, University of British Columbia, Vancouver, Canada.
J Biomed Mater Res. 1995 Mar;29(3):371-9. doi: 10.1002/jbm.820290312.
It is generally accepted that cell shape plays a pivotal role in many aspects of cell behavior including proliferation, differentiation, and gene expression. Although previous reports have shown that implant-surface topography can alter cell shape in vitro, in vivo evidence for such an effect is largely based on intuitive interpretation of two-dimensional histological sections. The objective of this in vivo study was to develop a method to reconstruct in three dimensions the shape of epithelial cells attached to smooth and micromachined implant surfaces. Titanium-coated epoxy replicas of smooth and 10-microns-deep micromachined grooved surfaces were implanted percutaneously in the parietal region of rats. After 7 days the implants and attached tissue were removed and processed for light and electron microscopy. One-micrometer-thick serial histological sections were used to trace and digitize cells and their nuclei into a Hewlett Packard computer. Three-dimensional images were reconstructed and rotated to measure length, width, height, area, orientation index and the angle cells or nuclei formed with the long axis of the grooves (XY angle), and the angle cells or nuclei formed with the long axis of the implant (XZ angle). Epithelial cells attached to the smooth surfaces were found to be significantly (P < .05) flatter and more spread than were the cells attached to the grooved surfaces. Cells on the smooth surfaces were aligned parallel with the long axis of the implant, whereas on the grooved surfaces cells were oriented obliquely with the implant.(ABSTRACT TRUNCATED AT 250 WORDS)
人们普遍认为，细胞形状在细胞行为的许多方面发挥着关键作用，包括增殖、分化和基因表达。尽管先前的报告表明植入物表面形貌可在体外改变细胞形状，但这种效应的体内证据很大程度上基于对二维组织学切片的直观解读。这项体内研究的目的是开发一种方法，以三维方式重建附着在光滑和微加工植入物表面的上皮细胞的形状。将光滑表面和10微米深的微加工沟槽表面的钛涂层环氧复制品经皮植入大鼠顶叶区域。7天后，取出植入物和附着组织，进行光镜和电镜处理。使用1微米厚的连续组织学切片将细胞及其细胞核进行追踪并数字化到惠普计算机中。重建三维图像并旋转以测量长度、宽度、高度、面积、取向指数以及细胞或细胞核与沟槽长轴形成的角度（XY角），以及细胞或细胞核与植入物长轴形成的角度（XZ角）。发现附着在光滑表面的上皮细胞比附着在沟槽表面的细胞明显更扁平且更伸展（P < 0.05）。光滑表面上的细胞与植入物的长轴平行排列，而沟槽表面上的细胞与植入物呈倾斜取向。（摘要截短于250字）
