Behar K L, Rothman D L, Hossmann K A
Department of Neurology, Yale University School of Medicine, New Haven, Connecticut 06510.
J Cereb Blood Flow Metab. 1989 Oct;9(5):655-65. doi: 10.1038/jcbfm.1989.93.
The effects of 1 h of complete global ischemia on the recovery of high-energy phosphates, intracellular pH (pHi), and lactate in the cat brain in vivo was investigated by 31P and 1H NMR spectroscopy. Ischemia led to a decrease in creatine phosphate (CrP), nucleoside triphosphates (NTP), and pHi, while inorganic phosphate and lactate increased. Intracellular pH decreased from a control value of 7.07 +/- 0.04 to 6.17 +/- 0.12 after 1 h of ischemia (N = 7). The degree of metabolic recovery after recirculation was variable. In three animals CrP and NTP were detected within 4 min and NTP increased to greater than or equal to 90% of control within 1 h; these levels were maintained for the 3 h of observation. In four other animals, CrP and NTP reached only 20 to 80% of control; however, high-energy phosphates decreased and lactate increased spontaneously between 1 and 2.5 h. Immediately following recirculation, pHi decreased further by an average of 0.3 units. The rate of recovery of cerebral pHi was slower than that of PCr and NTP for the majority of animals. Recovery of pHi was not detected for an average of 32 min after recirculation--by this time, NTP had attained 80 +/- 10% of their preischemic level. Recovery of pHi (and lactate) was not observed in two animals where PCr and NTP recovered transiently to only 30-43% of the preischemic level. Recovery of cerebral pHi was markedly heterogeneous in one animal, since two Pi peaks were detected shortly after recirculation.(ABSTRACT TRUNCATED AT 250 WORDS)
通过31P和1H核磁共振波谱法研究了1小时完全性全脑缺血对猫脑内高能磷酸盐、细胞内pH值（pHi）和乳酸恢复的影响。缺血导致磷酸肌酸（CrP）、核苷三磷酸（NTP）和pHi降低，而无机磷酸盐和乳酸增加。缺血1小时后，细胞内pH值从对照值7.07±0.04降至6.17±0.12（N = 7）。再灌注后代谢恢复的程度各不相同。在三只动物中，4分钟内检测到CrP和NTP，1小时内NTP增加至对照值的90%或更高；在观察的3小时内这些水平保持稳定。在另外四只动物中，CrP和NTP仅达到对照值的20%至80%；然而，在1至2.5小时之间高能磷酸盐自发降低，乳酸增加。再灌注后立即，pHi平均进一步降低0.3个单位。对于大多数动物，脑pHi的恢复速率比磷酸肌酸和NTP慢。再灌注后平均32分钟未检测到pHi的恢复——此时，NTP已达到缺血前水平的80±10%。在两只动物中未观察到pHi（和乳酸）的恢复，其中磷酸肌酸和NTP短暂恢复至缺血前水平的30 - 43%。在一只动物中脑pHi的恢复明显不均一，因为再灌注后不久检测到两个无机磷酸盐峰。（摘要截短于250字）
