Iwan-Zietek I, Ruszkowska-Ciastek B, Michalska M, Overskaug E, Goralczyk K, Dabrowiecki S, Rosc D
Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.
Division of Aesthetic Dermatology, Pomeranian Medical University in Szczecin, Szczecin, Poland.
J Physiol Pharmacol. 2016 Aug;67(4):555-561.
Adipokines regulate glucose homeostasis, insulin sensitivity, lipids metabolism, reproduction, as well as endothelial and platelets function. The study compares the plasma and adipose tissue concentrations of total adiponectin, leptin, leptin receptor and leptin-to-adiponectin ratio (LAR) in morbidly obese patients. Additionally it evaluates selected adipokines (leptin, adiponectin), endothelial markers and LAR depending on the gender in morbidly obese and non-obese subjects. The study involved 51 patients (31 women aged 21 - 60 (mean age of 39) and 20 men aged 24 to 60 (mean age of 41)). The eligibility criterion included the BMI ≥ 40 kg/m2. The non-obese group consisted of 30 healthy volunteers with the BMI < 24.9 kg/m2; nineteen women, aged 24 - 53 (mean age of 41), and 11 men aged 21 - 52 (mean age of 38). In the plasma and adipose tissue, the concentrations of total adiponectin, leptin, leptin receptor and plasma soluble forms of E-selectin, P-selectin, thrombomodulin were measured applying immunoassay techniques. There were noted significantly higher plasma leptin and sE-selectin concentrations, leptin-to-adiponectin ratio, additionally lower concentrations of plasma leptin receptor and sP-selectin in obese subjects regardless of the gender. Significantly higher concentrations of total adiponectin, leptin, leptin receptor expressed per 1 mg of total protein in adipose tissue, as compared to plasma in morbidly obese patients, were observed. Significant positive correlations between the BMI and the concentration of leptin and between total adiponectin and sP-selectin were reported in the subject group. Similarly there were noted significant negative correlations between leptin receptor and the BMI and between leptin-to-adiponectin ratio and sP-selectin in obese patients. The study has shown that adiponectin has a positive impact on platelets through a possible reduction in sP-selectin, and thus on platelets activation. On the other hand an elevated sE-selectin reveals perspective about the endothelium stimulation and a higher risk of endothelial damage in morbidly obese patients. Also in morbidly obese the higher leptin level and leptin-to-adiponectin ratio and simultaneously lower concentration of leptin receptor are associated with leptin resistance, additionally in possible future risk of insulin resistance and diabetes type 2.
脂肪因子调节葡萄糖稳态、胰岛素敏感性、脂质代谢、生殖以及内皮和血小板功能。该研究比较了病态肥胖患者血浆和脂肪组织中总脂联素、瘦素、瘦素受体以及瘦素与脂联素比值（LAR）的浓度。此外，该研究还根据性别评估了病态肥胖和非肥胖受试者中选定的脂肪因子（瘦素、脂联素）、内皮标志物和LAR。该研究纳入了51例患者（31名年龄在21至60岁之间（平均年龄39岁）的女性和20名年龄在24至60岁之间（平均年龄41岁）的男性）。入选标准为BMI≥40kg/m²。非肥胖组由30名BMI<24.9kg/m²的健康志愿者组成；19名年龄在24至53岁之间（平均年龄41岁）的女性和11名年龄在21至52岁之间（平均年龄38岁）的男性。采用免疫分析技术测定血浆和脂肪组织中总脂联素、瘦素、瘦素受体以及E选择素、P选择素、血栓调节蛋白的血浆可溶性形式的浓度。结果发现，无论性别，肥胖受试者的血浆瘦素和可溶性E选择素浓度、瘦素与脂联素比值均显著升高，此外血浆瘦素受体和可溶性P选择素浓度降低。与病态肥胖患者的血浆相比，观察到每1mg总蛋白中脂肪组织中总脂联素、瘦素、瘦素受体的浓度显著更高。在研究对象组中，BMI与瘦素浓度之间以及总脂联素与可溶性P选择素之间存在显著正相关。同样，在肥胖患者中，瘦素受体与BMI之间以及瘦素与脂联素比值与可溶性P选择素之间存在显著负相关。该研究表明，脂联素可能通过降低可溶性P选择素对血小板产生积极影响，从而影响血小板激活。另一方面，可溶性E选择素升高表明病态肥胖患者存在内皮刺激和内皮损伤风险增加的情况。此外，在病态肥胖患者中，较高的瘦素水平和瘦素与脂联素比值以及同时较低的瘦素受体浓度与瘦素抵抗有关，此外还可能存在未来胰岛素抵抗和2型糖尿病的风险。
