Jadaud P, Wainer I W
St. Jude Children's Research Hospital, Memphis, TN 38105.
J Chromatogr. 1989 Aug 4;476:165-74. doi: 10.1016/s0021-9673(01)93866-x.
A series of 24 enantiomeric and diastereomeric dipeptides were chromatographed on a chiral stationary phase (CSP) based upon immobilized alpha-chymotrypsin (ACHT). The ACHT-CSP was able to resolve stereochemically a number of the enantiomeric D,D- and L,L-dipeptides as well as the diastereomeric D,D-/L,L- and L,D-/D,L-dipeptides. The solutes were also chromatographed on a N-tosyl-L-phenylalanine chloromethyl ketone-deactivated form of the ACHT-CSP, where stereochemical separations were also achieved. The results of this study suggest that binding interactions between the dipeptides and the ACHT-CSP occur at the active site of the ACHT and at other hydrophobic sites on the ACHT molecule.
一系列24种对映体和非对映体二肽在基于固定化α-胰凝乳蛋白酶（ACHT）的手性固定相（CSP）上进行了色谱分析。ACHT-CSP能够立体化学拆分多种对映体D,D-和L,L-二肽以及非对映体D,D-/L,L-和L,D-/D,L-二肽。这些溶质还在ACHT-CSP的N-甲苯磺酰-L-苯丙氨酸氯甲基酮失活形式上进行了色谱分析，在那里也实现了立体化学分离。本研究结果表明，二肽与ACHT-CSP之间的结合相互作用发生在ACHT的活性位点以及ACHT分子上的其他疏水位点。
