Bi Cong, Zheng Xiwei, Azaria Shiden, Beeram Sandya, Li Zhao, Hage David S
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Separations. 2016 Sep;3(3). doi: 10.3390/separations3030027. Epub 2016 Sep 5.
The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used as binding agents to create chiral stationary phases (CSPs) and in the use of chromatographic methods to study these materials and protein-based chiral separations. The supports and methods that have been employed to prepare protein-based CSPs will also be discussed and compared. Specific types of CSPs that are considered include those that employ serum transport proteins (e.g., human serum albumin, bovine serum albumin, and alpha-acid glycoprotein), enzymes (e.g., penicillin G acylase, cellobiohydrolases, and α-chymotrypsin) or other types of proteins (e.g., ovomucoid, antibodies, and avidin or streptavidin). The properties and applications for each type of protein and CSP will also be discussed in terms of their use in chromatography and chiral separations.
用于分析和拆分手性药物及溶质的分离方法的发展一直是药物研究中一个持续受到关注的领域。在高效液相色谱（HPLC）中使用蛋白质作为手性结合剂是此类工作中备受特别关注的一种方法。本报告概述了已被用作结合剂以创建手性固定相（CSP）的蛋白质，以及在使用色谱方法研究这些材料和基于蛋白质的手性分离方面的情况。还将讨论和比较用于制备基于蛋白质的CSP的载体和方法。所考虑的特定类型的CSP包括那些使用血清转运蛋白（例如人血清白蛋白、牛血清白蛋白和α-酸性糖蛋白）、酶（例如青霉素G酰化酶、纤维二糖水解酶和α-胰凝乳蛋白酶）或其他类型蛋白质（例如卵类粘蛋白、抗体以及抗生物素蛋白或链霉抗生物素蛋白）的CSP。还将根据每种蛋白质和CSP在色谱和手性分离中的用途讨论其性质和应用。
