通过高效液相色谱法分离卟啉异构体。

Separation of porphyrin isomers by high-performance liquid chromatography.

作者信息

Kennedy S W, Maslen A L

机构信息

Canadian Wildlife Service, National Wildlife Research Centre, Ottawa, Ontario.

出版信息

J Chromatogr. 1989 Aug 25;493(1):53-62. doi: 10.1016/s0378-4347(00)82708-5.

DOI:10.1016/s0378-4347(00)82708-5

PMID:2778022

Abstract

A high-speed reversed-phase high-performance liquid chromatographic method using an octadecylsilyl 3 cm long (3 microns particle size) column to separate the free acids of uroporphyrins I and III and coproporphyrins I and III from each other, and from the type I isomers of several other porphyrin carboxylic acids, is described. Separation of the porphyrins was achieved in less than 8 min, and injections were possible every 12 min. The detection limits of uroporphyrin, coproporphyrin, and mesoporphyrin were 75, 45, and 35 fmol (at a signal-to-noise ratio of 2), respectively. Application of the method to the determination of urinary and liver porphyrin patterns is shown.

摘要

描述了一种高速反相高效液相色谱法，该方法使用一根3厘米长（粒径3微米）的十八烷基硅烷柱，可将尿卟啉I和III以及粪卟啉I和III的游离酸彼此分离，并与其他几种卟啉羧酸的I型异构体分离。卟啉的分离在不到8分钟内即可完成，且每12分钟可进样一次。尿卟啉、粪卟啉和中卟啉的检测限分别为75、45和35飞摩尔（信噪比为2时）。展示了该方法在测定尿和肝卟啉图谱中的应用。