通过高效液相色谱法从生物组织中提取和分离尿卟啉和粪卟啉异构体。

Extraction and isolation by high performance liquid chromatography of uroporphyrin and coproporphyrin isomers from biological tissues.

作者信息

Reddy V R, Christenson W R, Piper W N

出版信息

J Pharmacol Methods. 1987 Mar;17(1):51-7. doi: 10.1016/0160-5402(87)90036-2.

Abstract

A simple, rapid procedure has been developed for extraction of uroporphyrin and coproporphyrin isomers from biological tissues. The recoveries of known standards of uroporphyrin I and III and coproporphyrin I and III were performed from liver, kidney, testis, and bone marrow of the rat. The extracted samples were analyzed by high performance liquid chromatography. This method is suitable for the study of drug- and toxicant-induced porphyrias characterized by alterations of the ratios of the I and III isomers of uroporphyrin and coproporphyrin.

摘要

已开发出一种从生物组织中提取尿卟啉和粪卟啉异构体的简单、快速方法。从大鼠的肝脏、肾脏、睾丸和骨髓中对已知标准品尿卟啉I和III以及粪卟啉I和III进行回收率测定。提取的样品通过高效液相色谱法进行分析。该方法适用于研究以尿卟啉和粪卟啉I和III异构体比例改变为特征的药物和毒物诱导的卟啉症。