Kennedy S W, Wigfield D C, Fox G A
Anal Biochem. 1986 Aug 15;157(1):1-7. doi: 10.1016/0003-2697(86)90187-9.
A rapid and sensitive method for the determination of porphyrin carboxylic acids in liver, kidney, and spleen by high-speed high-performance liquid chromatography is described. Porphyrins were extracted with recoveries greater than or equal to 98%, concentrated on disposable octadecylsilyl cartridges, and analyzed with a liquid chromatograph equipped with a 3 microns X 3 cm octadecylsilyl column and a fluorescence detector. Separation of di-, tetra-, penta-, hexa-, hepta-, and octacarboxylic acids was achieved within 5 min. The detection limits for uro, copro, and protoporphyrin were 20, 10, and 20 fmol, respectively.
描述了一种通过高速高效液相色谱法测定肝脏、肾脏和脾脏中卟啉羧酸的快速灵敏方法。卟啉的提取回收率大于或等于98%，浓缩于一次性十八烷基硅烷柱上，并使用配备3微米×3厘米十八烷基硅烷柱和荧光检测器的液相色谱仪进行分析。在5分钟内实现了二羧酸、四羧酸、五羧酸、六羧酸、七羧酸和八羧酸的分离。尿卟啉、粪卟啉和原卟啉的检测限分别为20、10和20飞摩尔。
