一种用于检测和定量抗猪水疱病病毒（SVDV）抗体的酶联免疫吸附测定（ELISA）。

An enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of antibodies against swine vesicular disease virus (SVDV).

作者信息

Armstrong R M, Barnett I T

机构信息

AFRC Institute for Animal Health, Pirbright Laboratory, Woking, U.K.

出版信息

J Virol Methods. 1989 Jul;25(1):71-9. doi: 10.1016/0166-0934(89)90101-8.

DOI:10.1016/0166-0934(89)90101-8

PMID:2778030

Abstract

A liquid phase blocking sandwich ELISA has been compared with virus neutralisation for testing pig sera for antibodies against swine vesicular disease (SVD) virus. Highest infectivity titre of SVD virus was obtained using a multiplicity of infection of 30 pfu/cell and harvesting after 21 h. Titres obtained for 300 clinically normal animals were assessed by ELISA and 89% were found to be 1/6 or less. Results were skewed and spread up to 1/45. Comparison of known positive sera resulted in a correlation between the two methods of 0.68 and showed that a virus neutralisation titre of 1/16 was equivalent to 1/40 (log10 1.61) by ELISA. Variation in results obtained by replicate testing using ELISA and virus neutralisation was almost identical. Overlap between positive and negative sera was shown to be reduced to 1-1/2 fold in ELISA. Therefore, the ELISA correlated well with virus neutralisation and has several advantages over the latter.

摘要

已将液相阻断夹心酶联免疫吸附测定（ELISA）与病毒中和试验进行比较，以检测猪血清中抗猪水疱病（SVD）病毒的抗体。使用感染复数为30个空斑形成单位（pfu）/细胞并在21小时后收获，可获得SVD病毒的最高感染滴度。通过ELISA评估了300只临床正常动物的滴度，发现89%的滴度为1/6或更低。结果呈偏态分布，范围高达1/45。对已知阳性血清的比较表明，两种方法之间的相关性为0.68，并且显示病毒中和滴度为1/16相当于ELISA法的1/40（log10 1.61）。使用ELISA和病毒中和试验进行重复检测所获得结果的差异几乎相同。ELISA显示阳性和阴性血清之间的重叠减少至1 - 1/2倍。因此，ELISA与病毒中和试验相关性良好，并且比后者具有多个优点。