De Clercq K
Veterinary and Agrochemical Research Centre, Brussels (Ukkel), Belgium.
J Virol Methods. 1998 Jan;70(1):7-18. doi: 10.1016/s0166-0934(97)00164-x.
Pigs which are serologically positive for swine vesicular disease virus (SVDV) but which show no clinical signs and for which there is neither a relevant history of the disease on the holding nor contact with a known outbreak are considered as singleton reactors. False positive serological results for an epizootic disease, like SVD, in a non-vaccinated population or in imported animals are of great concern to international trade. For the virus neutralisation test, the gold standard for SVD, singleton reactors are found at a level of 1-3/1000. Singleton reactors also occur when other serological testing methods are used. The number of animals finally considered as singleton reactors can be reduced considerably by performing three different serological tests (virus neutralisation test, monoclonal antibody-based competitive ELISA and isotype specific ELISA) on the same serum. A serological profile of the animal can be derived by analysing the results in greater detail. This procedure can reduce considerably the number of pig holdings on which a prohibition of movement and trade needs to be imposed without requiring analysis of supplementary samples.
血清学检测对猪水疱病病毒（SVDV）呈阳性但无临床症状，且所在养殖场无相关疾病病史、未接触已知疫情爆发的猪，被视为单例反应动物。在未接种疫苗的群体或进口动物中，像猪水疱病这样的动物流行病出现假阳性血清学结果，对国际贸易来说是重大问题。对于病毒中和试验（猪水疱病的金标准），单例反应动物的出现比例为1 - 3/1000。使用其他血清学检测方法时也会出现单例反应动物。通过对同一血清进行三种不同的血清学检测（病毒中和试验、基于单克隆抗体的竞争ELISA和同型特异性ELISA），最终被视为单例反应动物的数量可大幅减少。通过更详细地分析结果，可以得出动物的血清学特征。此程序可大幅减少需要实施行动和贸易禁令的猪场数量，而无需分析补充样本。
