• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

源自宫颈癌的间充质基质细胞会产生大量腺苷以抑制细胞毒性T淋巴细胞功能。

Mesenchymal stromal cells derived from cervical cancer produce high amounts of adenosine to suppress cytotoxic T lymphocyte functions.

作者信息

de Lourdes Mora-García María, García-Rocha Rosario, Morales-Ramírez Omar, Montesinos Juan José, Weiss-Steider Benny, Hernández-Montes Jorge, Ávila-Ibarra Luis Roberto, Don-López Christian Azucena, Velasco-Velázquez Marco Antonio, Gutiérrez-Serrano Vianey, Monroy-García Alberto

机构信息

Immunobiology Laboratory, Cellular Differentiation and Cancer Unit, FES-Zaragoza, UNAM, Mexico City, Mexico.

Immunology and Cancer Laboratory, Oncology Research Unit, Oncology Hospital, National Medical Center, IMSS, Mexico City, Mexico.

出版信息

J Transl Med. 2016 Oct 26;14(1):302. doi: 10.1186/s12967-016-1057-8.

DOI:10.1186/s12967-016-1057-8
PMID:27782859
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5080842/
Abstract

BACKGROUND

In recent years, immunomodulatory mechanisms of mesenchymal stem/stromal cells (MSCs) from bone marrow and other "classic" sources have been described. However, the phenotypic and functional properties of tumor MSCs are poorly understood. The aim of this study was to analyze the immunosuppressive capacity of cervical cancer-derived MSCs (CeCa-MSCs) on effector T lymphocytes through the purinergic pathway.

METHODS

We determined the expression and functional activity of the membrane-associated ectonucleotidases CD39 and CD73 on CeCa-MSCs and normal cervical tissue-derived MSCs (NCx-MSCs). We also analyzed their immunosuppressive capacity to decrease proliferation, activation and effector cytotoxic T (CD8+) lymphocyte function through the generation of adenosine (Ado).

RESULTS

We detected that CeCa-MSCs express higher levels of CD39 and CD73 ectonucleotidases in cell membranes compared to NCx-MSCs, and that this feature was associated with the ability to strongly suppress the proliferation, activation and effector functions of cytotoxic T-cells through the generation of large amounts of Ado from the hydrolysis of ATP, ADP and AMP nucleotides.

CONCLUSIONS

This study suggests that CeCa-MSCs play an important role in the suppression of the anti-tumor immune response in CeCa through the purinergic pathway.

摘要

背景

近年来，已对来自骨髓和其他“经典”来源的间充质干/基质细胞（MSC）的免疫调节机制进行了描述。然而，肿瘤来源的MSC的表型和功能特性仍知之甚少。本研究旨在通过嘌呤能途径分析宫颈癌来源的MSC（CeCa-MSC）对效应T淋巴细胞的免疫抑制能力。

方法

我们测定了CeCa-MSC和正常宫颈组织来源的MSC（NCx-MSC）上膜相关外切核苷酸酶CD39和CD73的表达及功能活性。我们还分析了它们通过生成腺苷（Ado）来降低增殖、激活及效应细胞毒性T（CD8+）淋巴细胞功能的免疫抑制能力。

结果

我们检测到，与NCx-MSC相比，CeCa-MSC在细胞膜上表达更高水平的CD39和CD73外切核苷酸酶，并且这一特征与通过将ATP、ADP和AMP核苷酸水解生成大量Ado来强烈抑制细胞毒性T细胞的增殖、激活及效应功能的能力相关。

结论

本研究表明，CeCa-MSC通过嘌呤能途径在宫颈癌的抗肿瘤免疫反应抑制中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/318b23ccda23/12967_2016_1057_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/38d63c48103e/12967_2016_1057_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/c80137a8d691/12967_2016_1057_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/35a4831e72e0/12967_2016_1057_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/ecdbf7e1f595/12967_2016_1057_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/ae4f8be85299/12967_2016_1057_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/e6b42e01f929/12967_2016_1057_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/318b23ccda23/12967_2016_1057_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/38d63c48103e/12967_2016_1057_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/c80137a8d691/12967_2016_1057_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/35a4831e72e0/12967_2016_1057_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/ecdbf7e1f595/12967_2016_1057_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/ae4f8be85299/12967_2016_1057_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/e6b42e01f929/12967_2016_1057_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4fb3/5080842/318b23ccda23/12967_2016_1057_Fig7_HTML.jpg

相似文献

1
Mesenchymal stromal cells derived from cervical cancer produce high amounts of adenosine to suppress cytotoxic T lymphocyte functions.源自宫颈癌的间充质基质细胞会产生大量腺苷以抑制细胞毒性T淋巴细胞功能。
J Transl Med. 2016 Oct 26;14(1):302. doi: 10.1186/s12967-016-1057-8.
2
Adenosinergic Immunosuppression by Human Mesenchymal Stromal Cells Requires Co-Operation with T cells.人骨髓间充质基质细胞的腺苷能免疫抑制需要与T细胞合作。
Stem Cells. 2016 Mar;34(3):781-90. doi: 10.1002/stem.2280. Epub 2016 Jan 26.
3
Mesenchymal stromal cells derived from cervical cancer tumors induce TGF-β1 expression and IL-10 expression and secretion in the cervical cancer cells, resulting in protection from cytotoxic T cell activity.源自宫颈癌肿瘤的间充质基质细胞可诱导宫颈癌细胞中转化生长因子-β1（TGF-β1）的表达以及白细胞介素-10（IL-10）的表达和分泌，从而使宫颈癌细胞免受细胞毒性T细胞活性的影响。
Cytokine. 2015 Dec;76(2):382-390. doi: 10.1016/j.cyto.2015.09.001. Epub 2015 Sep 3.
4
Cervical cancer cells suppress effector functions of cytotoxic T cells through the adenosinergic pathway.宫颈癌细胞通过腺苷能途径抑制细胞毒性T细胞的效应功能。
Cell Immunol. 2017 Oct;320:46-55. doi: 10.1016/j.cellimm.2017.09.002. Epub 2017 Sep 7.
5
Mesenchymal Stromal Cells Derived from Normal Cervix and Cervical Cancer Tumors Increase CD73 Expression in Cervical Cancer Cells Through TGF-β1 Production.正常宫颈和宫颈癌肿瘤来源的间充质基质细胞通过 TGF-β1 的产生增加宫颈癌细胞中的 CD73 表达。
Stem Cells Dev. 2019 Apr 1;28(7):477-488. doi: 10.1089/scd.2018.0183. Epub 2019 Mar 5.
6
Mesenchymal stromal cells up-regulate CD39 and increase adenosine production to suppress activated T-lymphocytes.间充质基质细胞上调CD39并增加腺苷生成以抑制活化的T淋巴细胞。
Stem Cell Res. 2011 Jul;7(1):66-74. doi: 10.1016/j.scr.2011.04.001. Epub 2011 Apr 8.
7
In vitro evidence of the presence of mesenchymal stromal cells in cervical cancer and their role in protecting cancer cells from cytotoxic T cell activity.体外证据表明宫颈癌中存在间充质基质细胞，并且这些细胞在保护癌细胞免受细胞毒性 T 细胞活性方面发挥作用。
Stem Cells Dev. 2013 Sep 15;22(18):2508-19. doi: 10.1089/scd.2013.0084. Epub 2013 Jun 14.
8
Adenosine increases PD-L1 expression in mesenchymal stromal cells derived from cervical cancer through its interaction with AR/AR and the production of TGF-β1.腺苷通过与 AR/AR 相互作用和产生 TGF-β1 增加宫颈癌来源的间充质基质细胞中 PD-L1 的表达。
Cell Biochem Funct. 2024 Apr;42(3):e4010. doi: 10.1002/cbf.4010.
9
Mesenchymal Stem/Stromal Cells Derived from Dental Tissues Mediate the Immunoregulation of T Cells through the Purinergic Pathway.牙髓组织来源的间充质干细胞通过嘌呤能途径介导 T 细胞的免疫调节。
Int J Mol Sci. 2024 Sep 4;25(17):9578. doi: 10.3390/ijms25179578.
10
Reply: Adenosine Producing Mesenchymal Stromal Cells.回复：产生腺苷的间充质基质细胞。
Stem Cells. 2017 Jun;35(6):1649-1650. doi: 10.1002/stem.2531. Epub 2016 Nov 15.

引用本文的文献

1
Tumor-associated mesenchymal stem/stromal cells in tumor microenvironment and carcinogenesis.肿瘤微环境中的肿瘤相关间充质干细胞与肿瘤发生
Exp Hematol Oncol. 2025 Jul 17;14(1):97. doi: 10.1186/s40164-025-00688-7.
2
Isolation and Characterization of Cervical Cancer-Associated Mesenchymal Stem Cells From Primary Tumors Using Explant Culture.利用组织块培养法从原发性肿瘤中分离并鉴定宫颈癌相关间充质干细胞
Bio Protoc. 2025 Jun 20;15(12):e5358. doi: 10.21769/BioProtoc.5358.
3
TGF-β Induces the Secretion of Extracellular Vesicles Enriched with CD39 and CD73 from Cervical Cancer Cells.

本文引用的文献

1
Anti-CD73 in cancer immunotherapy: awakening new opportunities.癌症免疫治疗中的抗CD73：开启新机遇
Trends Cancer. 2016 Feb 1;2(2):95-109. doi: 10.1016/j.trecan.2016.01.003.
2
Adenosinergic Immunosuppression by Human Mesenchymal Stromal Cells Requires Co-Operation with T cells.人骨髓间充质基质细胞的腺苷能免疫抑制需要与T细胞合作。
Stem Cells. 2016 Mar;34(3):781-90. doi: 10.1002/stem.2280. Epub 2016 Jan 26.
3
CD73 Pathway Contributes to the Immunosuppressive Ability of Mesenchymal Stem Cells in Intraocular Autoimmune Responses.
转化生长因子-β诱导宫颈癌细胞分泌富含CD39和CD73的细胞外囊泡。
Int J Mol Sci. 2025 Mar 7;26(6):2413. doi: 10.3390/ijms26062413.
4
Adenosinergic Signalling in Cervical Cancer Microenvironment.宫颈癌微环境中的腺苷能信号传导
Expert Rev Mol Med. 2025 Jan 7;27:e5. doi: 10.1017/erm.2024.30.
5
Mesenchymal Stem/Stromal Cells Derived from Dental Tissues Mediate the Immunoregulation of T Cells through the Purinergic Pathway.牙髓组织来源的间充质干细胞通过嘌呤能途径介导 T 细胞的免疫调节。
Int J Mol Sci. 2024 Sep 4;25(17):9578. doi: 10.3390/ijms25179578.
6
Inhibition of insulin-like growth factors increases production of CXCL9/10 by macrophages and fibroblasts and facilitates CD8 cytotoxic T cell recruitment to pancreatic tumours.抑制胰岛素样生长因子会增加巨噬细胞和成纤维细胞产生 CXCL9/10，并促进 CD8 细胞毒性 T 细胞向胰腺肿瘤的募集。
Front Immunol. 2024 Aug 5;15:1382538. doi: 10.3389/fimmu.2024.1382538. eCollection 2024.
7
Revisiting the role of mesenchymal stromal cells in cancer initiation, metastasis and immunosuppression.重新审视间充质基质细胞在癌症起始、转移和免疫抑制中的作用。
Exp Hematol Oncol. 2024 Jul 1;13(1):64. doi: 10.1186/s40164-024-00532-4.
8
Glycan-based scaffolds and nanoparticles as drug delivery system in cancer therapy.基于聚糖的支架和纳米颗粒作为癌症治疗中的药物传递系统。
Front Immunol. 2024 May 10;15:1395187. doi: 10.3389/fimmu.2024.1395187. eCollection 2024.
9
Adenosine Increases the Immunosuppressive Capacity of Cervical Cancer Cells by Increasing PD-L1 Expression and TGF-β Production through Its Interaction with AR/AR.腺苷通过与AR/AR相互作用增加PD-L1表达和TGF-β产生，从而增强宫颈癌细胞的免疫抑制能力。
Pharmaceuticals (Basel). 2024 Mar 19;17(3):397. doi: 10.3390/ph17030397.
10
Engineered Adoptive T-Cell Therapies for Breast Cancer: Current Progress, Challenges, and Potential.用于乳腺癌的工程化过继性T细胞疗法：当前进展、挑战与潜力
Cancers (Basel). 2023 Dec 26;16(1):124. doi: 10.3390/cancers16010124.
CD73通路在眼内自身免疫反应中有助于间充质干细胞的免疫抑制能力。
Stem Cells Dev. 2016 Feb 15;25(4):337-46. doi: 10.1089/scd.2015.0227. Epub 2016 Jan 29.
4
Immunomodulation of endothelial differentiated mesenchymal stromal cells: impact on T and NK cells.内皮分化间充质基质细胞的免疫调节：对T细胞和自然杀伤细胞的影响。
Immunol Cell Biol. 2016 Apr;94(4):342-56. doi: 10.1038/icb.2015.94. Epub 2015 Oct 29.
5
Mesenchymal stromal cells derived from cervical cancer tumors induce TGF-β1 expression and IL-10 expression and secretion in the cervical cancer cells, resulting in protection from cytotoxic T cell activity.源自宫颈癌肿瘤的间充质基质细胞可诱导宫颈癌细胞中转化生长因子-β1（TGF-β1）的表达以及白细胞介素-10（IL-10）的表达和分泌，从而使宫颈癌细胞免受细胞毒性T细胞活性的影响。
Cytokine. 2015 Dec;76(2):382-390. doi: 10.1016/j.cyto.2015.09.001. Epub 2015 Sep 3.
6
Multipotent Mesenchymal Stromal Cells: Possible Culprits in Solid Tumors?多能间充质基质细胞：实体瘤的可能罪魁祸首？
Stem Cells Int. 2015;2015:914632. doi: 10.1155/2015/914632. Epub 2015 Jul 27.
7
Role of IL-10 and TGF-β1 in local immunosuppression in HPV-associated cervical neoplasia.白细胞介素-10和转化生长因子-β1在人乳头瘤病毒相关宫颈肿瘤局部免疫抑制中的作用
World J Clin Oncol. 2014 Oct 10;5(4):753-63. doi: 10.5306/wjco.v5.i4.753.
8
Role of TGF-β signaling in generation of CD39+CD73+ myeloid cells in tumors.转化生长因子-β信号在肿瘤中CD39⁺CD73⁺髓样细胞生成中的作用。
J Immunol. 2014 Sep 15;193(6):3155-64. doi: 10.4049/jimmunol.1400578. Epub 2014 Aug 15.
9
The roles of CD73 in cancer.CD73在癌症中的作用。
Biomed Res Int. 2014;2014:460654. doi: 10.1155/2014/460654. Epub 2014 Jul 14.
10
Adenosine uptake is the major effector of extracellular ATP toxicity in human cervical cancer cells.腺苷摄取是人类宫颈癌细胞中细胞外ATP毒性的主要效应因素。
Mol Biol Cell. 2014 Oct 1;25(19):2905-18. doi: 10.1091/mbc.E14-01-0042. Epub 2014 Aug 7.