Sun Hong-Zhi, Meng Fan-Jie, Guo Heng-Yue
Department of Laboratorial Medicine,Yidu Central Hospital of Weifang City, Qingzhou 262500, Shandong Province, China. E-mail:
Department of Laboratorial Medicine,Yidu Central Hospital of Weifang City, Qingzhou 262500, Shandong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Oct;24(5):1319-1323. doi: 10.7534/j.issn.1009-2137.2016.05.006.
To explore the gene expression of insulin-like growth factor binding protein 3 (IGFBP3) in bone marrow mononuclear cells and the expression of IGFBP3 in peripheral blood as well their significance.
A total of 178 patients with acute myeloid leukemia (AML) from March 2014 to March 2016 were divided into de novo AML, CR, and relapse groups according to their condition; Patients with non-hematologic malignancies and normal bone marrow in the same period were selected as control group. The ELISA method was used to detect the IGFBP3 protein levels in peripheral blood, and PCR method were used to detecte IGFBP3 gene expression in bone marrow mono-nucleated cells.
IGFBP3 gene expression levels of bone marrow mononuclear cells in de novo AML and relapse groups were significantly lower than that in control group (P<0.05), while that in CR group and control group, de novo AML and relapse groups was no significantly different (P>0.05); IGFBP3 levels of peripheral blood in de novo AML and relapse groups were significantly lower than those in control group (P<0.05), while those in CR group and control group, de novo AML and relapse groups were no significantly different (P>0.05); IGFBP3 expression levels in peripheral blood and bone marrow mononucleated cells did not show significant correlation.
The gene expression of IGFBP3 in bone marrow mononuclear cells and its protein levels in peripheral blood may play an important role in occurrence and development of acute myeloid leukemia, and it can also evaluate the disease status and treatment efficacy of acute myeloid leukemia in some extent.
探讨胰岛素样生长因子结合蛋白3（IGFBP3）在骨髓单个核细胞中的基因表达及在外周血中的表达情况及其意义。
选取2014年3月至2016年3月期间的178例急性髓系白血病（AML）患者，根据病情分为初发AML组、完全缓解（CR）组和复发组；选取同期非血液系统恶性肿瘤患者及正常骨髓者作为对照组。采用ELISA法检测外周血中IGFBP3蛋白水平，采用PCR法检测骨髓单个核细胞中IGFBP3基因表达。
初发AML组和复发组骨髓单个核细胞IGFBP3基因表达水平明显低于对照组（P<0.05），而CR组与对照组、初发AML组与复发组比较差异无统计学意义（P>0.05）；初发AML组和复发组外周血IGFBP3水平明显低于对照组（P<0.05），而CR组与对照组、初发AML组与复发组比较差异无统计学意义（P>0.05）；外周血与骨髓单个核细胞中IGFBP3表达水平无明显相关性。
IGFBP3在骨髓单个核细胞中的基因表达及其在外周血中的蛋白水平可能在急性髓系白血病的发生发展中起重要作用，且在一定程度上可评估急性髓系白血病的病情及治疗效果。
