Liu Lin, Liao Zi-Long, Yan Xiao-Jing, Liu Jie, Wang Xiao-Ou, Fan Hua
Department of Hematology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Department of Hematology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):935-9. doi: 10.7534/j.issn.1009-2137.2015.04.005.
To explore the expression of insulin-like growth factor binding protein 3 (IGFBP3) in acute myeloid leukemia and its clinical significance.
Using GAPDH as internal reference, IGFBP3 gene expression was detected in the bone marrow mononuclear cells of 43 de novo AML patients, 36 patients with complete remission (CR) and 28 cases of controls by using SYBR-Green I real-time quantitative PCR, the differences of gene expression between the three groups were compared.
IGFBP3 gene expression level in the de novo group were lower than that in CR group and control group (P < 0.05), but there was no statistically significant difference of IGFBP3 expression level in CR group and control group (P > 0.05).
The IGFBP3 as a tumor suppressor gene may play a role in the pathogenesis of acute myeloid leukemia, its expression level is recovered after complete remission, therefore, IGFBP3 can be used as an indicator of evaluating clinical curative effect.
探讨胰岛素样生长因子结合蛋白3（IGFBP3）在急性髓系白血病中的表达及其临床意义。
以甘油醛-3-磷酸脱氢酶（GAPDH）为内参，采用SYBR-Green I实时定量聚合酶链反应（PCR）检测43例初发急性髓系白血病患者、36例完全缓解（CR）患者及28例对照组骨髓单个核细胞中IGFBP3基因的表达，比较三组基因表达差异。
初发组IGFBP3基因表达水平低于CR组和对照组（P<0.05），但CR组与对照组IGFBP3表达水平差异无统计学意义（P>0.05）。
IGFBP3作为一种抑癌基因可能在急性髓系白血病发病机制中发挥作用，完全缓解后其表达水平恢复，因此，IGFBP3可作为评估临床疗效的指标。
