利用双相硫酸钙/羟基磷灰石生物材料，肌肉作为局部骨形成的骨诱导微环境。
Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial.
作者信息
Raina D B, Gupta A, Petersen M M, Hettwer W, McNally M, Tägil M, Zheng M-H, Kumar A, Lidgren L
机构信息
Department of Orthopaedics, Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, 208016, UP, India.
Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur, 208016, UP, India.
出版信息
Bone Joint Res. 2016 Oct;5(10):500-511. doi: 10.1302/2046-3758.510.BJR-2016-0133.R1.
OBJECTIVES
We have observed clinical cases where bone is formed in the overlaying muscle covering surgically created bone defects treated with a hydroxyapatite/calcium sulphate biomaterial. Our objective was to investigate the osteoinductive potential of the biomaterial and to determine if growth factors secreted from local bone cells induce osteoblastic differentiation of muscle cells.
MATERIALS AND METHODS
We seeded mouse skeletal muscle cells C2C12 on the hydroxyapatite/calcium sulphate biomaterial and the phenotype of the cells was analysed. To mimic surgical conditions with leakage of extra cellular matrix (ECM) proteins and growth factors, we cultured rat bone cells ROS 17/2.8 in a bioreactor and harvested the secreted proteins. The secretome was added to rat muscle cells L6. The phenotype of the muscle cells after treatment with the media was assessed using immunostaining and light microscopy.
RESULTS
C2C12 cells differentiated into osteoblast-like cells expressing prominent bone markers after seeding on the biomaterial. The conditioned media of the ROS 17/2.8 contained bone morphogenetic protein-2 (BMP-2 8.4 ng/mg, standard deviation (sd) 0.8) and BMP-7 (50.6 ng/mg, sd 2.2). In vitro, this secretome induced differentiation of skeletal muscle cells L6 towards an osteogenic lineage.
CONCLUSION
Extra cellular matrix proteins and growth factors leaking from a bone cavity, along with a ceramic biomaterial, can synergistically enhance the process of ectopic ossification. The overlaying muscle acts as an osteoinductive niche, and provides the required cells for bone formation.Cite this article: D. B. Raina, A. Gupta, M. M. Petersen, W. Hettwer, M. McNally, M. Tägil, M-H. Zheng, A. Kumar, L. Lidgren. Muscle as an osteoinductive niche for local bone formation with the use of a biphasic calcium sulphate/hydroxyapatite biomaterial. Bone Joint Res 2016;5:500-511. DOI: 10.1302/2046-3758.510.BJR-2016-0133.R1.
目的
我们观察到在用羟基磷灰石/硫酸钙生物材料治疗手术造成的骨缺损时，覆盖肌肉中形成骨的临床病例。我们的目的是研究该生物材料的骨诱导潜力，并确定局部骨细胞分泌的生长因子是否能诱导肌肉细胞向成骨细胞分化。
材料与方法
我们将小鼠骨骼肌细胞C2C12接种在羟基磷灰石/硫酸钙生物材料上，并分析细胞的表型。为模拟细胞外基质（ECM）蛋白和生长因子泄漏的手术条件，我们在生物反应器中培养大鼠骨细胞ROS 17/2.8，并收集分泌的蛋白质。将分泌蛋白添加到大鼠肌肉细胞L6中。使用免疫染色和光学显微镜评估用该培养基处理后肌肉细胞的表型。
结果
C2C12细胞接种在生物材料上后分化为表达显著骨标志物的成骨样细胞。ROS 17/2.8的条件培养基含有骨形态发生蛋白-2（BMP-2 8.4 ng/mg，标准差（sd）0.8）和BMP-7（50.6 ng/mg，sd 2.2）。在体外，这种分泌蛋白诱导骨骼肌细胞L6向成骨谱系分化。
结论
从骨腔泄漏的细胞外基质蛋白和生长因子，与陶瓷生物材料一起，可协同增强异位骨化过程。覆盖的肌肉充当骨诱导微环境，并为骨形成提供所需的细胞。引用本文：D. B. Raina，A. Gupta，M. M. Petersen，W. Hettwer，M. McNally，M. Tägil，M-H. Zheng，A. Kumar，L. Lidgren。利用双相硫酸钙/羟基磷灰石生物材料，肌肉作为局部骨形成的骨诱导微环境。骨关节研究2016；5：500 - 511。DOI：10.1302/2046 - 3758.510.BJR - 2016 - 0133.R1 。
Zotero 插件