Clancy Neil T, Saso Srdjan, Stoyanov Danail, Sauvage Vincent, Corless David J, Boyd Michael, Noakes David E, Thum Meen-Yau, Ghaem-Maghami Sadaf, Smith James Richard, Elson Daniel S
Imperial College London, Institute of Global Health Innovation, Hamlyn Centre for Robotic Surgery, London SW7 2AZ, United KingdombImperial College London, Division of Surgery, Department of Surgery and Cancer, London SW7 2AZ, United Kingdom.
Imperial College London, Hammersmith Hospital Campus, Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology, London W12 0NN, United Kingdom.
J Biomed Opt. 2016 Oct 1;21(10):106006. doi: 10.1117/1.JBO.21.10.106006.
Uterine transplantation surgery (UTx) has been proposed as a treatment for permanent absolute uterine factor infertility (AUFI) in the case of the congenital absence or surgical removal of the uterus. Successful surgical attachment of the organ and its associated vasculature is essential for the organ’s reperfusion and long-term viability. Spectral imaging techniques have demonstrated the potential for the measurement of hemodynamics in medical applications. These involve the measurement of reflectance spectra by acquiring images of the tissue in different wavebands. Measures of tissue constituents at each pixel can then be extracted from these spectra through modeling of the light–tissue interaction. A multispectral imaging (MSI) laparoscope was used in sheep and rabbit UTx models to study short- and long-term changes in oxygen saturation following surgery. The whole organ was imaged in the donor and recipient animals in parallel with point measurements from a pulse oximeter. Imaging results confirmed the re-establishment of adequate perfusion in the transplanted organ after surgery. Cornual oxygenation trends measured with MSI are consistent with pulse oximeter readings, showing decreased StO2 immediately after anastomosis of the blood vessels. Long-term results show recovery of StO2 to preoperative levels.
子宫移植手术（UTx）已被提议作为治疗因先天性子宫缺失或手术切除子宫导致的永久性绝对子宫因素不孕症（AUFI）的一种方法。成功地将器官及其相关血管进行手术连接对于器官的再灌注和长期存活至关重要。光谱成像技术已证明在医学应用中测量血流动力学的潜力。这些技术涉及通过获取不同波段的组织图像来测量反射光谱。然后可以通过对光与组织相互作用进行建模，从这些光谱中提取每个像素处的组织成分测量值。在绵羊和兔子的子宫移植模型中使用多光谱成像（MSI）腹腔镜来研究手术后短期和长期的氧饱和度变化。在供体和受体动物中对整个器官进行成像，并与脉搏血氧仪的点测量同时进行。成像结果证实了手术后移植器官中充分灌注的重新建立。用MSI测量的子宫角氧合趋势与脉搏血氧仪读数一致，显示血管吻合后立即出现StO2降低。长期结果显示StO2恢复到术前水平。
