Kengelbach-Weigand Annika, Lotz Laura, Schmid Rafael, Lang Werner, Beckmann Matthias W, Hoffmann Inge, Horch Raymund E, Renner Stefan P, Dittrich Ralf, Boos Anja M, Hildebrandt Thomas
Department of Plastic and Hand Surgery, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen (FAU), Erlangen, Germany
Department of Obstetrics and Gynecology, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen (FAU), Erlangen, Germany.
In Vivo. 2019 Mar-Apr;33(2):325-336. doi: 10.21873/invivo.11478.
The introduction of the opportunity to transplant a viable uterus into women for fulfilling their desire to have a child has awakened high expectations worldwide.
A sheep model was used to evaluate tools for optimizing measurement of blood flow in uterine transplantation. Intraoperatively, blood flow was measured using unidirectional Doppler and indocyanine green (ICG) fluorescence imaging. Postoperatively, an implantable Doppler probe served as a tool for clinical monitoring the patency of anastomosed vessels.
ICG imaging showed complete vascularization of the uterus before and in short-term evaluation after surgery. The implantable Doppler probe proved to be highly suitable for assessing patency of vessels in a non-invasive way. Results of histology, and real-time polymerase chain reaction demonstrated viability of the transplanted uterus.
Different methods to monitor vasculature patency have proven to be advantageous in supporting both surgeons and researchers in ensuring successful implementation of uterine transplantation.
为满足女性生育愿望而向其移植有活力子宫这一机会的出现，在全球范围内引发了很高的期望。
使用绵羊模型评估优化子宫移植中血流测量的工具。术中，使用单向多普勒和吲哚菁绿（ICG）荧光成像测量血流。术后，可植入式多普勒探头作为临床监测吻合血管通畅性的工具。
ICG成像显示术前及术后短期评估时子宫完全血管化。可植入式多普勒探头被证明非常适合以非侵入性方式评估血管通畅性。组织学和实时聚合酶链反应结果证明移植子宫具有活力。
已证明不同的监测血管系统通畅性的方法有利于支持外科医生和研究人员确保子宫移植的成功实施。
