Center for Bionanoengineering and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.
Adv Mater. 2016 Dec;28(48):10613-10622. doi: 10.1002/adma.201603095. Epub 2016 Oct 27.
Selective gene expression in tumors via responsive dissociation of polyplexes triggered by intracellular signals is demonstrated. An esterase-responsive charge-reversal polymer mediates selective gene expression in the cancer cells high in esterases over fibroblasts low in esterase activity. Its gene therapy with the TRAIL suicide gene effectively induces apoptosis of HeLa cells but does not activate fibroblasts to secrete WNT16B, enabling potent cancer gene therapy with few side effects.
通过细胞内信号触发的多聚物的响应性解离来实现肿瘤中的选择性基因表达。一种酯酶响应性荷质转变聚合物介导了在酯酶活性高的癌细胞中而非在酯酶活性低的成纤维细胞中的选择性基因表达。用 TRAIL 自杀基因进行其基因治疗有效地诱导了 HeLa 细胞的凋亡，但不会激活成纤维细胞分泌 WNT16B，从而能够进行副作用少的有效的癌症基因治疗。
