Kodogo Vitaris, Zhou Danai Tavonga, Oektedalen Olav, Duri Kerina, Stray-Pedersen Babill, Gomo Exnevia
Department of Medical Laboratory Sciences, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe.
Department of Medical Laboratory Sciences, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe; Institute of Clinical Medicine, Oslo University Hospital, Oslo, Norway.
Open AIDS J. 2016 Sep 30;10:190-198. doi: 10.2174/1874613601610010190. eCollection 2016.
Dyslipidemia does not occur in all HIV-infected or antiretroviral therapy-experienced patients suggesting role of host genetic factors but there is paucity of data on association between dyslipidemia and gene polymorphisms in Zimbabwe.
To determine association of lipoprotein levels and polymorphisms in HIV-infected adults.
Demographic data were collected from 103 consenting patients; lipoprotein levels were determined and blood samples were successfully genotyped for both 2488C>T Xba1 and 4154G>A p.Gln4154Lys EcoR1 polymorphisms by real time polymerase chain reaction.
Mean age of genotyped patients was 40.3 ± 10.1 years, 68% were female; prevalence of dyslipidemia was 67.4%. Of 103 samples genotyped for Xba1 polymorphism, 76 (74%) were homozygous C/C, 24 (23%) were heterozygous C/T and only three (3%) were homozygous T/T. EcoR1 polymorphism showed little variability, one participant had rare genotype A/A, 68.3% had wild type genotype G/G.
Observed frequencies of XbaI and EcoRI polymorphisms matched other African studies. In spite of low numbers of rare variants, there was positive association between both total cholestrol and high density lipoprotein with ECoR1 wild type G/G genotype, suggesting that ECoRI 4154 G allele could be more protective against coronary heart disease than EcoR1 4154 A allele. There is need for further research at population level to confirm whether ECoR1 genotyping is useful for predicting risk of dyslipidemia in HIV patients in our setting.
并非所有感染人类免疫缺陷病毒（HIV）或接受过抗逆转录病毒治疗的患者都会出现血脂异常，这表明宿主遗传因素起到了一定作用，但在津巴布韦，关于血脂异常与基因多态性之间关联的数据却很匮乏。
确定HIV感染成人中脂蛋白水平与基因多态性之间的关联。
收集了103名同意参与研究的患者的人口统计学数据；测定了脂蛋白水平，并通过实时聚合酶链反应成功对2488C>T Xba1和4154G>A p.Gln4154Lys EcoR1这两种基因多态性进行了血液样本基因分型。
进行基因分型的患者平均年龄为40.3±10.1岁，68%为女性；血脂异常患病率为67.4%。在针对Xba1基因多态性进行基因分型的103个样本中，76个（74%）为纯合子C/C，24个（23%）为杂合子C/T，只有3个（3%）为纯合子T/T。EcoR1基因多态性显示出的变异性较小，1名参与者具有罕见基因型A/A，68.3%具有野生型基因型G/G。
观察到的XbaI和EcoRI基因多态性频率与其他非洲研究结果相符。尽管罕见变异数量较少，但总胆固醇和高密度脂蛋白与EcoR1野生型G/G基因型之间均存在正相关，这表明EcoR1 4154 G等位基因可能比EcoR1 4154 A等位基因对冠心病具有更强的保护作用。有必要在人群层面进行进一步研究，以确认EcoR1基因分型在我们的研究环境中是否有助于预测HIV患者血脂异常的风险。
