Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Ciências da Saúde, Universidade de Brasília, Brasília, DF, Brasil.
Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade de Brasília, Brasília, DF, Brasil.
Braz J Med Biol Res. 2020 Mar 2;53(3):e9102. doi: 10.1590/1414-431X20199102. eCollection 2020.
The apolipoprotein B (APOB) gene contains several polymorphic sites described as risk modifiers for cardiovascular events. The objective of this study was to verify the association of the classic APOB Xba I polymorphism (rs693) with atherosclerotic risk factors in a segment of the Brazilian elderly population considering their usual dietary intake. Clinical and biochemical characteristics as well as total caloric and fat intake data were determined from 644 elderly individuals. Polymorphism analysis was performed by conventional polymerase chain reaction followed by enzyme restriction. Statistical analyses compared measures and proportions according to different APOB genotypic combinations. Statistically significant association was found between Xba I polymorphism and serum LDL, total cholesterol, and total lipid levels, with important elevations among T homozygotes compared to the other genotypes. There was homogeneity in all other parameters analyzed (including intake pattern), with a tendency for reduced levels of circulating apolipoprotein B among TT individuals. Our results pointed out that genetic variation in APOB affected the lipemic profile of elderly individuals in a context not biased by diet, generating a pattern suggestive of secretory disorder of lipoprotein particles, with possible implication in atherosclerotic risk.
载脂蛋白 B（APOB）基因包含多个多态性位点，这些位点被描述为心血管事件的风险修饰因子。本研究的目的是验证经典 APOB Xba I 多态性（rs693）与巴西老年人群体动脉粥样硬化风险因素的关联，同时考虑他们的常规饮食摄入。从 644 名老年人中确定了临床和生化特征以及总热量和脂肪摄入量数据。通过常规聚合酶链反应（PCR）后酶切进行多态性分析。根据不同的 APOB 基因型组合比较了测量值和比例的统计分析。Xba I 多态性与血清 LDL、总胆固醇和总脂质水平之间存在统计学显著相关性，与其他基因型相比，T 纯合子的升高更为显著。在所有其他分析的参数（包括摄入模式）中都具有同质性，与 TT 个体相比，循环载脂蛋白 B 的水平呈降低趋势。我们的结果表明，APOB 中的遗传变异影响了老年人群的血脂谱，而这种影响不受饮食的影响，产生了脂蛋白颗粒分泌紊乱的模式，可能与动脉粥样硬化风险有关。
