• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

经典Wnt信号通路在骨骼肌终末分化过程中调节Setdb1的核输出。

Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation.

作者信息

Beyer Sophie, Pontis Julien, Schirwis Elija, Battisti Valentine, Rudolf Anja, Le Grand Fabien, Ait-Si-Ali Slimane

机构信息

Centre National de la Recherche Scientifique CNRS-Université Paris Diderot, Sorbonne Paris Cité, Epigenetics and Cell Fate UMR7216 , Paris, France.

Institut Cochin, Université Paris-Descartes, Centre National de la Recherche Scientifique (CNRS) UMR8104, Paris, France; Institut National de la Santé et de la Recherche Médicale (INSERM) U1016, Paris, France.

出版信息

Cell Discov. 2016 Oct 18;2:16037. doi: 10.1038/celldisc.2016.37. eCollection 2016.

DOI:10.1038/celldisc.2016.37
PMID:27790377
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5067623/
Abstract

The histone 3 lysine 9 methyltransferase Setdb1 is essential for both stem cell pluripotency and terminal differentiation of different cell types. To shed light on the roles of Setdb1 in these mutually exclusive processes, we used mouse skeletal myoblasts as a model of terminal differentiation. studies on isolated single myofibres showed that Setdb1 is required for adult muscle stem cells expansion following activation. studies in skeletal myoblasts confirmed that Setdb1 suppresses terminal differentiation. Genomic binding analyses showed a release of Setdb1 from selected target genes upon myoblast terminal differentiation, concomitant to a nuclear export of Setdb1 to the cytoplasm. Both genomic release and cytoplasmic Setdb1 relocalisation during differentiation were dependent on canonical Wnt signalling. Transcriptomic assays in myoblasts unravelled a significant overlap between Setdb1 and Wnt3a regulated genetic programmes. Together, our findings revealed Wnt-dependent subcellular relocalisation of Setdb1 as a novel mechanism regulating Setdb1 functions and myogenesis.

摘要

组蛋白3赖氨酸9甲基转移酶Setdb1对于干细胞多能性和不同细胞类型的终末分化都至关重要。为了阐明Setdb1在这些相互排斥的过程中的作用，我们使用小鼠骨骼肌成肌细胞作为终末分化的模型。对分离出的单个肌纤维的研究表明，Setdb1是成年肌肉干细胞激活后增殖所必需的。对骨骼肌成肌细胞的研究证实，Setdb1抑制终末分化。基因组结合分析表明，成肌细胞终末分化时，Setdb1从选定的靶基因上释放，同时Setdb1向细胞质进行核输出。分化过程中的基因组释放和细胞质Setdb1重新定位均依赖于经典Wnt信号通路。对成肌细胞的转录组分析揭示了Setdb1和Wnt3a调控的遗传程序之间存在显著重叠。总之，我们的研究结果揭示了Setdb1依赖Wnt的亚细胞重新定位是一种调节Setdb1功能和肌生成的新机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/75d7574dc037/celldisc201637-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/3f97ae44bd8f/celldisc201637-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/ba02ff3eaba4/celldisc201637-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/890870c3caa1/celldisc201637-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/0e5c01b23682/celldisc201637-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/0163b5099672/celldisc201637-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/75d7574dc037/celldisc201637-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/3f97ae44bd8f/celldisc201637-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/ba02ff3eaba4/celldisc201637-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/890870c3caa1/celldisc201637-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/0e5c01b23682/celldisc201637-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/0163b5099672/celldisc201637-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d69a/5067623/75d7574dc037/celldisc201637-f6.jpg

相似文献

1
Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation.经典Wnt信号通路在骨骼肌终末分化过程中调节Setdb1的核输出。
Cell Discov. 2016 Oct 18;2:16037. doi: 10.1038/celldisc.2016.37. eCollection 2016.
2
Erratum: Canonical Wnt signalling regulates nuclear export of Setdb1 during skeletal muscle terminal differentiation.勘误：经典Wnt信号通路在骨骼肌终末分化过程中调节Setdb1的核输出。
Cell Discov. 2016 Nov 15;2:16043. doi: 10.1038/celldisc.2016.43. eCollection 2016.
3
Wnt/β-catenin controls follistatin signalling to regulate satellite cell myogenic potential.Wnt/β-catenin 调控卵泡抑素信号以调节卫星细胞的成肌潜能。
Skelet Muscle. 2015 Apr 28;5:14. doi: 10.1186/s13395-015-0038-6. eCollection 2015.
4
In silico probing and biological evaluation of SETDB1/ESET-targeted novel compounds that reduce tri-methylated histone H3K9 (H3K9me3) level.对降低三甲基化组蛋白H3K9（H3K9me3）水平的SETDB1/ESET靶向新型化合物的计算机模拟研究和生物学评估。
J Comput Aided Mol Des. 2017 Oct;31(10):877-889. doi: 10.1007/s10822-017-0052-3. Epub 2017 Sep 6.
5
Ubiquitination of Lysine 867 of the Human SETDB1 Protein Upregulates Its Histone H3 Lysine 9 (H3K9) Methyltransferase Activity.人类SETDB1蛋白赖氨酸867位点的泛素化上调其组蛋白H3赖氨酸9（H3K9）甲基转移酶活性。
PLoS One. 2016 Oct 31;11(10):e0165766. doi: 10.1371/journal.pone.0165766. eCollection 2016.
6
Regulated nuclear entry of over-expressed Setdb1.过表达 Setdb1 的调控核内输入。
Genes Cells. 2013 Aug;18(8):694-703. doi: 10.1111/gtc.12068. Epub 2013 Jun 20.
7
SETDB1 in cancer: overexpression and its therapeutic implications.癌症中的SETDB1：过表达及其治疗意义。
Am J Cancer Res. 2021 May 15;11(5):1803-1827. eCollection 2021.
8
KMT1E mediated H3K9 methylation is required for the maintenance of embryonic stem cells by repressing trophectoderm differentiation.KMT1E 介导的 H3K9 甲基化通过抑制滋养外胚层分化来维持胚胎干细胞。
Stem Cells. 2010 Feb;28(2):201-12. doi: 10.1002/stem.278.
9
Functional dissection of N-terminal nuclear trafficking signals of SETDB1.SETDB1的N端核转运信号的功能剖析
Front Cell Dev Biol. 2022 Dec 20;10:1069765. doi: 10.3389/fcell.2022.1069765. eCollection 2022.
10
The H3K9 Methylation Writer SETDB1 and its Reader MPP8 Cooperate to Silence Satellite DNA Repeats in Mouse Embryonic Stem Cells.H3K9 甲基化写入器 SETDB1 及其读取器 MPP8 合作沉默小鼠胚胎干细胞中的卫星 DNA 重复序列。
Genes (Basel). 2019 Sep 25;10(10):750. doi: 10.3390/genes10100750.

引用本文的文献

1
The SUV39 Family of H3K9 Methyltransferases in Skeletal Muscle Stem Cells.骨骼肌干细胞中H3K9甲基转移酶的SUV39家族
FASEB Bioadv. 2025 May 3;7(6):e70014. doi: 10.1096/fba.2024-00102. eCollection 2025 Jun.
2
PHF2 regulates sarcomeric gene transcription in myogenesis.PHF2在肌生成过程中调节肌节基因转录。
PLoS One. 2024 May 3;19(5):e0301690. doi: 10.1371/journal.pone.0301690. eCollection 2024.
3
SETDB1 modulates the TGFβ response in Duchenne muscular dystrophy myotubes.SETDB1 调节杜氏肌营养不良症肌管中的 TGFβ 反应。

本文引用的文献

1
β-Catenin Activation in Muscle Progenitor Cells Regulates Tissue Repair.肌肉祖细胞中的β-连环蛋白激活调节组织修复。
Cell Rep. 2016 May 10;15(6):1277-90. doi: 10.1016/j.celrep.2016.04.022. Epub 2016 Apr 28.
2
Analysis of the subcellular localization of the human histone methyltransferase SETDB1.人类组蛋白甲基转移酶SETDB1的亚细胞定位分析。
Biochem Biophys Res Commun. 2015 Oct 2;465(4):725-31. doi: 10.1016/j.bbrc.2015.08.065. Epub 2015 Aug 18.
3
SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells.
Sci Adv. 2024 May 3;10(18):eadj8042. doi: 10.1126/sciadv.adj8042. Epub 2024 May 1.
4
Cytoplasmic localization of SETDB1‑induced Warburg effect via c‑MYC‑LDHA axis enhances migration and invasion in breast carcinoma.SETDB1 诱导的 Warburg 效应通过 c-MYC-LDHA 轴导致的细胞质定位增强了乳腺癌的迁移和侵袭。
Int J Mol Med. 2024 Apr;53(4). doi: 10.3892/ijmm.2024.5364. Epub 2024 Mar 1.
5
Cell-type differential targeting of SETDB1 prevents aberrant CTCF binding, chromatin looping, and cis-regulatory interactions.细胞类型特异性靶向 SETDB1 可防止异常 CTCF 结合、染色质环化和顺式调控相互作用。
Nat Commun. 2024 Jan 2;15(1):15. doi: 10.1038/s41467-023-44578-0.
6
The functions of SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) in biological process and disease.SETDB1 结构域分裂组蛋白赖氨酸甲基转移酶 1（SETDB1）在生物过程和疾病中的功能。
Epigenetics Chromatin. 2023 Dec 7;16(1):47. doi: 10.1186/s13072-023-00519-1.
7
The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells.组蛋白赖氨酸甲基转移酶Setdb1的细胞质部分对胚胎干细胞至关重要。
iScience. 2023 Jul 14;26(8):107386. doi: 10.1016/j.isci.2023.107386. eCollection 2023 Aug 18.
8
Histone methyltransferase SETDB1 promotes osteogenic differentiation in osteoporosis by activating OTX2-mediated BMP-Smad and Wnt/β-catenin pathways.组蛋白甲基转移酶 SETDB1 通过激活 OTX2 介导的 BMP-Smad 和 Wnt/β-catenin 通路促进骨质疏松症中的成骨分化。
Hum Cell. 2023 Jul;36(4):1373-1388. doi: 10.1007/s13577-023-00902-w. Epub 2023 Apr 19.
9
Binary Colloidal Crystals Promote Cardiac Differentiation of Human Pluripotent Stem Cells via Nuclear Accumulation of SETDB1.二元胶体晶体通过 SETDB1 的核积累促进人多能干细胞的心脏分化。
ACS Nano. 2023 Feb 14;17(3):3181-3193. doi: 10.1021/acsnano.3c00009. Epub 2023 Jan 19.
10
SETDB1 regulates microtubule dynamics.SETDB1 调控微管动态。
Cell Prolif. 2022 Dec;55(12):e13348. doi: 10.1111/cpr.13348. Epub 2022 Nov 3.
在小鼠胚胎干细胞中，SETDB1独立于H3K9三甲基化调控发育基因处的PRC2活性。
Genome Res. 2015 Sep;25(9):1325-35. doi: 10.1101/gr.177576.114. Epub 2015 Jul 9.
4
Wnt/β-catenin controls follistatin signalling to regulate satellite cell myogenic potential.Wnt/β-catenin 调控卵泡抑素信号以调节卫星细胞的成肌潜能。
Skelet Muscle. 2015 Apr 28;5:14. doi: 10.1186/s13395-015-0038-6. eCollection 2015.
5
A KAP1 phosphorylation switch controls MyoD function during skeletal muscle differentiation.一种KAP1磷酸化开关在骨骼肌分化过程中控制MyoD功能。
Genes Dev. 2015 Mar 1;29(5):513-25. doi: 10.1101/gad.254532.114.
6
Setdb1 is required for myogenic differentiation of C2C12 myoblast cells via maintenance of MyoD expression.Setdb1通过维持MyoD表达，对C2C12成肌细胞的肌源性分化是必需的。
Mol Cells. 2015 Apr;38(4):362-72. doi: 10.14348/molcells.2015.2291. Epub 2015 Feb 25.
7
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.使用DESeq2对RNA测序数据的倍数变化和离散度进行适度估计。
Genome Biol. 2014;15(12):550. doi: 10.1186/s13059-014-0550-8.
8
The SWI/SNF subunit/tumor suppressor BAF47/INI1 is essential in cell cycle arrest upon skeletal muscle terminal differentiation.SWI/SNF亚基/肿瘤抑制因子BAF47/INI1在骨骼肌终末分化时的细胞周期停滞中至关重要。
PLoS One. 2014 Oct 1;9(10):e108858. doi: 10.1371/journal.pone.0108858. eCollection 2014.
9
AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene.AGO2和SETDB1协同作用于雄激素受体基因启动子靶向的转录沉默。
Nucleic Acids Res. 2014 Dec 16;42(22):13545-56. doi: 10.1093/nar/gku788. Epub 2014 Sep 2.
10
Suv39h-dependent H3K9me3 marks intact retrotransposons and silences LINE elements in mouse embryonic stem cells.Suv39h 依赖性 H3K9me3 标记完整的反转录转座子，并使小鼠胚胎干细胞中的 LINE 元件沉默。
Mol Cell. 2014 Jul 17;55(2):277-90. doi: 10.1016/j.molcel.2014.05.029. Epub 2014 Jun 26.