Belay Tesfaye, Fu Chih-Lung, Woart Anthony
School of Arts and Sciences, Bluefield State College, Bluefield, WV 24701, USA.
Current Address is the National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA.
J Nutr Med Diet Care. 2015;1(1). doi: 10.23937/2572-3278.1510006. Epub 2015 Sep 15.
A cold-induced stress mouse model for investigating chlamydia genital infection and immune response analysis was established in our laboratory. Previous results showed that cold-induced stress results in suppression of the immune response and increased intensity of chlamydia genital infection in the mouse model. The purpose of the present study was to evaluate the potential therapeutic value of active hexose correlated compound (AHCC) against chlamydia genital infection in mice. AHCC is an extract of mushroom commonly used as a dietary supplement is known to boost the immune system. Mice were infected intravaginally with after a 24-day cold-stress application. Oral administration of AHCC to stressed or non-stressed mice was carried out seven days before infection and during the course of infection along with cervicovaginal swabbing. Cytokine production by peritoneal and splenic T cells isolated from AHCC-fed stressed mice and non-stressed mice was measured ELISA. Splenic T cells from both animal groups were co-cultured with mouse monocyte J774.2 cell line or cultured by addition of supernatants of AHCC-treated J774.2 cell line for 24 hours. Infection studies showed that AHCC-feeding compared to phosphate buffered saline (PBS)-feeding to stressed mice resulted in reduced shedding from the genital tract. Levels of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) were significantly increased in stressed mice receiving AHCC compared to stressed mice receiving PBS. Production of interferon gamma (IFN-γ) and interleukin 2 (IL-2) in the AHCC group was significantly high compared to production in PBS-fed group. Splenic T cells from stressed and non-stressed cultured with supernatants of AHCC-treated J774.2 cell line resulted in significantly increased TNF-α or IFN-γ production. Results obtained in this study show that AHCC improves the function of immune cells as indicated by the restoration of levels of cytokines production that were suppressed under cold induced-stress conditions. This is the first report showing that oral administration of AHCC enhances the function of the immune system, which could result in increased resistance of the host to chlamydia genital infection.
我们实验室建立了一种用于研究衣原体生殖道感染和免疫反应分析的冷诱导应激小鼠模型。先前的结果表明，在该小鼠模型中，冷诱导应激会导致免疫反应受到抑制，衣原体生殖道感染强度增加。本研究的目的是评估活性己糖相关化合物（AHCC）对小鼠衣原体生殖道感染的潜在治疗价值。AHCC是一种蘑菇提取物，常用作膳食补充剂，已知可增强免疫系统。在施加24天冷应激后，小鼠经阴道感染[此处原文缺失感染物相关信息]。在感染前7天以及感染期间，对受应激或未受应激的小鼠进行AHCC口服给药，并同时进行宫颈阴道拭子检查。通过酶联免疫吸附测定法（ELISA）测量从喂食AHCC的受应激小鼠和未受应激小鼠中分离出的腹膜和脾脏T细胞产生的细胞因子。将两组动物的脾脏T细胞与小鼠单核细胞J774.2细胞系共培养，或通过添加经AHCC处理的J774.2细胞系的上清液培养24小时。感染研究表明，与喂食磷酸盐缓冲盐水（PBS）的受应激小鼠相比，喂食AHCC的受应激小鼠生殖道衣原体脱落减少。与接受PBS的受应激小鼠相比，接受AHCC的受应激小鼠中肿瘤坏死因子-α（TNF-α）和白细胞介素6（IL-6）水平显著升高。与PBS喂养组相比，AHCC组中干扰素γ（IFN-γ）和白细胞介素2（IL-2）的产生显著更高。用经AHCC处理的J774.2细胞系的上清液培养受应激和未受应激的脾脏T细胞，导致TNF-α或IFN-γ产生显著增加。本研究获得的结果表明，AHCC可改善免疫细胞功能，这表现为在冷诱导应激条件下被抑制的细胞因子产生水平得以恢复。这是首次报道口服AHCC可增强免疫系统功能，这可能导致宿主对衣原体生殖道感染的抵抗力增强。
