Roch Toralf, Kratz Karl, Ma Nan, Lendlein Andreas
Institute of Biomaterial Science and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, Germany.
Helmholtz Virtual Institute - Multifunctional Biomaterials for Medicine, Teltow and Berlin, Germany.
Clin Hemorheol Microcirc. 2016;64(4):899-910. doi: 10.3233/CH-168033.
Although frequently used as implants materials, both polydimethylsiloxane (PDMS) and polytetrafluoroethylene (PTFE) are often associated with adverse effects including foreign body responses. Dendritic cells (DC) are crucial for the initiation of immune reactions and could also play a role in foreign body associated inflammations. Therefore, the interaction of DC with PDMS and PTFE was investigated regarding their capacity to induce undesired cell activation. Medical grade PDMS and PTFE films were embedded into polystyrene PS inserts via injection molding to prevent the DC from migrating below the substrate and thereby, interacting not only with the test sample but also with the culture vessel material. The viability, the expression of co-stimulatory molecules, and the cytokine/chemokine profiles were determined after 24 hours incubation of the DC with PDMS or PTFE. Blank PS inserts and tissue culture polystyrene (TCP) served as reference materials. The viability of DC was not substantially influenced after incubation with PDMS and PTFE. However, both polymers induced DC activation indicated by the upregulation of co-stimulatory molecules. The release profiles of 14 soluble inflammatory mediators showed substantial differences between PDMS, PTFE, PS, and TCP. This study showed the potential of PTFE and PDMS to activate primary human dendritic cells, which could be an explanation for the often observed inflammatory events associated with the implantation of these polymers.
尽管聚二甲基硅氧烷（PDMS）和聚四氟乙烯（PTFE）经常被用作植入材料，但它们常常与包括异物反应在内的不良反应相关联。树突状细胞（DC）对于免疫反应的启动至关重要，并且也可能在与异物相关的炎症中发挥作用。因此，研究了DC与PDMS和PTFE的相互作用，以了解它们诱导不期望的细胞活化的能力。通过注塑成型将医用级PDMS和PTFE薄膜嵌入聚苯乙烯（PS）插入物中，以防止DC迁移到基质下方，从而不仅与测试样品相互作用，还与培养容器材料相互作用。在DC与PDMS或PTFE孵育24小时后，测定其活力、共刺激分子的表达以及细胞因子/趋化因子谱。空白PS插入物和组织培养聚苯乙烯（TCP）用作参考材料。与PDMS和PTFE孵育后，DC的活力没有受到实质性影响。然而，两种聚合物都诱导了DC活化，这通过共刺激分子的上调得以表明。14种可溶性炎症介质的释放谱在PDMS、PTFE、PS和TCP之间显示出显著差异。这项研究表明PTFE和PDMS具有激活原代人树突状细胞的潜力，这可能是这些聚合物植入后经常观察到的炎症事件的一个解释。
