Biomedical Engineering, The University of South Dakota, 4800 N. Career Avenue, Sioux Falls, South Dakota, 57107.
Sanford Research, 2301 East 60th Street North, Sioux Falls, South Dakota, 57104.
J Biomed Mater Res A. 2017 Sep;105(9):2441-2450. doi: 10.1002/jbm.a.36099. Epub 2017 Jun 6.
Polytetrafluoroethylene (PTFE) is one of the commonly used materials in making various cardiovascular implants. However, the success rates of these implants in several occasions are hindered by unwanted immune responses from immune cells, such as macrophages. In this study, we investigated the response of macrophages with different structures (flat, expanded, and electrospun) of PTFE having varied surface topographies: smooth planar surface (flat PTFE), node-fibrils (ePTFE), and randomly oriented microfibers (electrospun PTFE). The electrospun PTFE showed the least adhesion of macrophages. Also, the morphology of macrophages adhered on electrospun PTFE exhibited minimal activation. The macrophage pro-inflammatory cytokine secretions showed that the lowest level of TNF-α was produced on electrospun PTFE whereas IP-10 was produced in lowest levels on expanded PTFE (ePTFE). The production of IL-6 and MCP-1 cytokines was also dependent on the structure of PTFE that the macrophages interacted with, but in a time-dependent manner. Confocal microscopy images taken at 7, 14, and 21 days showed that the electrospun PTFE resulted in the lowest percentage of macrophage fusion, thus indicating the least possible chance of foreign body giant cell (FBGC) formation. Therefore, this study showed that electrospun PTFE with randomly oriented microfibers can provide reduced adhesion, activation, and FBGC formation of macrophages compared to the smooth and planar surface of flat PTFE and node-fibril structured surface of ePTFE. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2441-2450, 2017.
聚四氟乙烯（PTFE）是用于制造各种心血管植入物的常用材料之一。然而，在某些情况下，这些植入物的成功率受到免疫细胞（如巨噬细胞）产生的不良免疫反应的阻碍。在这项研究中，我们研究了具有不同表面形貌的不同结构（平面、扩展和静电纺丝）的 PTFE 对巨噬细胞的反应：光滑平面（平面 PTFE）、节点纤维（ePTFE）和随机取向的微纤维（静电纺丝 PTFE）。静电纺丝 PTFE 显示出对巨噬细胞的最小粘附。此外，粘附在静电纺丝 PTFE 上的巨噬细胞形态表现出最小的激活。巨噬细胞促炎细胞因子的分泌表明，静电纺丝 PTFE 产生的 TNF-α 水平最低，而扩展 PTFE（ePTFE）产生的 IP-10 水平最低。与巨噬细胞相互作用的 PTFE 结构也依赖于细胞因子 IL-6 和 MCP-1 的产生，但具有时间依赖性。在第 7、14 和 21 天拍摄的共聚焦显微镜图像显示，静电纺丝 PTFE 导致巨噬细胞融合的百分比最低，因此表明形成异物巨细胞（FBGC）的可能性最小。因此，这项研究表明，与光滑和平坦的平面 PTFE 和节点纤维结构表面的 ePTFE 相比，具有随机取向微纤维的静电纺丝 PTFE 可减少巨噬细胞的粘附、激活和 FBGC 形成。© 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A：105A：2441-2450，2017。
