Ye Chenyi, Zhang Wei, Wang Shengdong, Jiang Shuai, Yu Yuanbin, Chen Erman, Xue Deting, Chen Jianzhong, He Rongxin
Department of Orthopedic Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, No. 88, Jiefang Road, Hangzhou 310009, China.
Orthopedics Research Institute of Zhejiang University, No. 88, Jiefang Road, Hangzhou 310009, China.
Int J Mol Sci. 2016 Oct 25;17(11):1780. doi: 10.3390/ijms17111780.
To investigate whether the systematic administration of icariin (ICA) promotes tendon-bone healing after rotator cuff reconstruction in vivo, a total of 64 male Sprague Dawley rats were used in a rotator cuff injury model and underwent rotator cuff reconstruction (bone tunnel suture fixation). Rats from the ICA group ( = 32) were gavage-fed daily with ICA at 0.125 mg/g, while rats in the control group ( = 32) received saline only. Micro-computed tomography, biomechanical tests, serum ELISA (calcium; Ca, alkaline phosphatase; AP, osteocalcin; OCN) and histological examinations (Safranin O and Fast Green staining, type I, II and III collagen (Col1, Col2, and Col3), CD31, and vascular endothelial growth factor (VEGF)) were analyzed two and four weeks after surgery. In the ICA group, the serum levels of AP and OCN were higher than in the control group. More Col1-, Col2-, CD31-, and VEGF-positive cells, together with a greater degree of osteogenesis, were detected in the ICA group compared with the control group. During mechanical testing, the ICA group showed a significantly higher ultimate failure load than the control group at both two and four weeks. Our results indicate that the systematic administration of ICA could promote angiogenesis and tendon-bone healing after rotator cuff reconstruction, with superior mechanical strength compared with the controls. Treatment for rotator cuff injury using systematically-administered ICA could be a promising strategy.
为了研究淫羊藿苷（ICA）的系统性给药是否能促进体内肩袖重建术后的腱骨愈合，本研究共使用64只雄性Sprague Dawley大鼠建立肩袖损伤模型，并进行肩袖重建（骨隧道缝合固定）。ICA组（n = 32）的大鼠每天以0.125 mg/g的剂量灌胃ICA，而对照组（n = 32）的大鼠仅接受生理盐水。在术后两周和四周对大鼠进行微型计算机断层扫描、生物力学测试、血清ELISA（钙；Ca、碱性磷酸酶；AP、骨钙素；OCN）以及组织学检查（番红O和固绿染色、I型、II型和III型胶原蛋白（Col1、Col2和Col3）、CD31以及血管内皮生长因子（VEGF））。在ICA组中，AP和OCN的血清水平高于对照组。与对照组相比，ICA组检测到更多的Col1、Col2、CD31和VEGF阳性细胞，同时成骨程度更高。在力学测试中，ICA组在术后两周和四周的极限破坏载荷均显著高于对照组。我们的结果表明，ICA的系统性给药可促进肩袖重建术后的血管生成和腱骨愈合，与对照组相比具有更高的力学强度。使用系统性给药的ICA治疗肩袖损伤可能是一种有前景的策略。
