AcSIR (Academy of Scientific and Innovative Research), CSIR-NEERI (National Environmental Engineering Research Institute) campus, Nagpur-440020, India; Environmental Health Division, CSIR-NEERI (National Environmental Engineering Research Institute), Nagpur 440020, India.
Environmental Health Division, CSIR-NEERI (National Environmental Engineering Research Institute), Nagpur 440020, India.
Bioresour Technol. 2017 Jan;223:184-191. doi: 10.1016/j.biortech.2016.10.043. Epub 2016 Oct 18.
Current study reports isolation of Cupriavidus strain a3 which can utilize 2-chloro-4-nitrophenol (C4NP) as sole source of carbon and nitrogen, leading to its detoxification. Degradation process was initiated by release of nitrite ion resulting in the formation of 2-chlorohydroquinone as intermediate. The nitrite releasing activity was also evident in the cell free protein extract. Different parameters for 2C4NP biodegradation were optimized. The degradation pattern followed Haldane substrate inhibition model with maximum specific degradation rate (q) of 0.13/h, half saturation constant (K) of 0.05mM, and 2C4NP inhibition constant (K) of 0.64mM. The isolate was successfully applied to remediation of 2C4NP-contaminated soil in microcosm study. 2-Dimensional protein electrophoresis analysis showed that growth of the isolate in the presence of 2C4NP resulted in modification of membrane permeability and induction of signal transduction protein. In our knowledge, this is the first study reporting degradation and detoxification of 2C4NP by Cupriavidus.
本研究报告了铜绿假单胞菌菌株 a3 的分离，该菌株可以利用 2-氯-4-硝基苯酚（C4NP）作为唯一的碳源和氮源，从而实现其解毒。降解过程由亚硝酸盐离子的释放引发，导致 2-氯氢醌的形成作为中间产物。细胞游离蛋白提取物中也存在亚硝酸盐释放活性。优化了 2C4NP 生物降解的不同参数。降解模式符合 Haldane 底物抑制模型，最大比降解速率（q）为 0.13/h，半饱和常数（K）为 0.05mM，2C4NP 抑制常数（K）为 0.64mM。该分离株成功应用于微宇宙研究中 2C4NP 污染土壤的修复。二维蛋白质电泳分析表明，该分离株在 2C4NP 存在下的生长导致膜通透性的改变和信号转导蛋白的诱导。据我们所知，这是首次报道铜绿假单胞菌对 2C4NP 的降解和解毒。
