小鼠植入前发育过程中合成的阶段特异性多肽的鉴定。

Levinson J, Goodfellow P, vadeboncoeur M, McDevitt H

Proc Natl Acad Sci U S A. 1978 Jul;75(7):3332-6. doi: 10.1073/pnas.75.7.3332.

[35S]Methionine-labeled extracts of mouse ova and preimplantation embryos were analyzed by two dimensional polyacrylamide gel electrophoresis. Of the 400-600 molecular species that have been resolved as distinct spots on autoradiograms of gels for every stage of development from unfertilized eggs to early blastocysts, particular attention has been paid to the identification of 36 of these proteins, each of which is expressed only for a portion of the period under investigation. These molecules are referred to as stage-specific polypeptides and they are biochemical markers of early embryonic development and differentiation.

用[35S]甲硫氨酸标记小鼠卵子和植入前胚胎的提取物，通过二维聚丙烯酰胺凝胶电泳进行分析。从未受精卵到早期囊胚的每个发育阶段，在凝胶放射自显影片上已分辨出400 - 600种不同的分子种类，其中特别关注了36种蛋白质的鉴定，这些蛋白质中的每一种仅在研究期间的一部分时间内表达。这些分子被称为阶段特异性多肽，它们是早期胚胎发育和分化的生化标志物。