Center of Reproductive Medicine, Affiliated Hospital of Guilin Medical College, Guilin, People's Republic of China.
Assisted Reproductive Technology Unit, Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong.
Fertil Steril. 2017 Jan;107(1):136-143.e2. doi: 10.1016/j.fertnstert.2016.09.016. Epub 2016 Oct 25.
To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC).
Observational cohort study.
University assisted reproductive unit.
PATIENT(S): Fifty women were recruited: 18 NC, 12 unexplained RIF, and 20 RM.
INTERVENTIONS(S): None.
MAIN OUTCOME MEASURE(S): Endometrial biopsy was precisely timed 7 days after LH surge. The expression of HOXA-10 and E-cadherin were examined by means of immunohistochemistry. H-Scores of staining intensity in the glandular epithelium and stroma were measured.
RESULT(S): HOXA-10 signal was mainly localized in the nuclei of stroma cells and the cytoplasm of glandular epithelium cells. E-Cadherin signal was found only in the cytoplasm of glandular epithelium cells. The HOXA-10 H-scores in the RIF group and the RM group were significantly lower than in the control group in both the glandular epithelium and stroma. The E-cadherin H-scores in the RM group were also significantly lower than in the control group. Interestingly, there was a positive correlation between HOXA-10 and E-cadherin H-scores in all of the women examined.
CONCLUSION(S): There is a positive correlation between levels of HOXA-10 and E-cadherin expression in the endometrium, both of which are significantly reduced in women with RIF and RM compared with fertile control women. The findings suggest a potential role of HOXA-10 and E-cadherin in the implantation processes and altered expression in women with reproductive failure.
比较复发性植入失败（RIF）、复发性流产（RM）和有生育能力的女性（正常对照组；NC）的子宫内膜中 HOXA-10 和 E-钙黏蛋白的表达。
观察性队列研究。
大学辅助生殖单位。
招募了 50 名女性：18 名 NC、12 名不明原因的 RIF 和 20 名 RM。
无。
在 LH 峰后 7 天精确进行子宫内膜活检。通过免疫组织化学检测 HOXA-10 和 E-钙黏蛋白的表达。测量腺体上皮和基质染色强度的 H 评分。
HOXA-10 信号主要定位于基质细胞的核和腺上皮细胞的细胞质。E-Cadherin 信号仅在腺上皮细胞的细胞质中发现。在 RIF 组和 RM 组中，HOXA-10 的 H 评分在腺体上皮和基质中均明显低于对照组。在 RM 组中，E-cadherin 的 H 评分也明显低于对照组。有趣的是，在所检查的所有女性中，HOXA-10 和 E-cadherin 的 H 评分之间存在正相关。
在子宫内膜中，HOXA-10 和 E-钙黏蛋白的表达呈正相关，与有生育能力的对照组女性相比，RIF 和 RM 女性的这两种表达均明显降低。这些发现表明 HOXA-10 和 E-cadherin 在植入过程中可能具有作用，并且在生殖失败的女性中表达发生改变。
