Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, Hong Kong.
Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
Reprod Biol Endocrinol. 2018 Jun 21;16(1):60. doi: 10.1186/s12958-018-0379-1.
It is postulated that women suffered from recurrent implantation failure (RIF) have different endometrial receptivity compared to those who experienced with idiopathic recurrent miscarriage (RM). In this study, expression of common endometrial markers Leukemia inhibitor factor (LIF), mucin 1 (MUC1) and integrin β3 were studied and compared.
Fourteen women with RIF, 25 with RM and 20 fertile controls were recruited for endometrial biopsy during implantation window on day LH + 7. Spatial and temporal expression of MUC1, LIF and Integrin β3 were compared using semi-quantitative immunohistochemistry. Association of MUC1, LIF and integrin β3 expression levels with demographic and clinical characteristics were determined.
MUC1 expression in both luminal and glandular epithelium in women with RIF were significantly lower than that in women with RM and fertile controls. There were no differences in LIF and Integrin β3 expression in endometrial epithelium among three groups. Decreased MUC1 expression were not significantly associated with age, BMI, gravidity, parity, cycle length, progesterone level and previous miscarriage.
Deceased expression of MUC1 is an independent marker for endometrial receptivity in RIF women, suggesting MUC1 may contribute to the reproductive failure in RIF women.
据推测，与经历特发性复发性流产（RM）的女性相比，反复着床失败（RIF）的女性的子宫内膜容受性不同。在这项研究中，研究了常见的子宫内膜标志物白血病抑制因子（LIF）、粘蛋白 1（MUC1）和整合素β3 的表达并进行了比较。
招募了 14 名 RIF 女性、25 名 RM 女性和 20 名生育能力正常的对照组，在 LH+7 天的着床窗口期进行子宫内膜活检。使用半定量免疫组织化学比较 MUC1、LIF 和整合素β3 的时空表达。确定 MUC1、LIF 和整合素β3 表达水平与人口统计学和临床特征的相关性。
RIF 女性的腔上皮和腺体上皮中的 MUC1 表达明显低于 RM 女性和生育能力正常的对照组。三组子宫内膜上皮中的 LIF 和整合素β3 表达无差异。MUC1 表达降低与年龄、BMI、孕次、产次、周期长度、孕酮水平和既往流产史无显著相关性。
MUC1 的表达降低是 RIF 女性子宫内膜容受性的独立标志物，表明 MUC1 可能导致 RIF 女性的生殖失败。
