Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Fertil Steril. 2017 Jan;107(1):167-173.e2. doi: 10.1016/j.fertnstert.2016.09.036. Epub 2016 Oct 25.
To characterize peritoneal dendritic cells (DCs) in endometriosis and to clarify their role in its etiology.
Experimental.
University hospital.
PATIENT(S): Sixty-three women (35 patients with endometriosis and 28 control women) who had undergone laparoscopic surgery.
INTERVENTION(S): Peritoneal DCs from endometriosis and control samples were analyzed for the expression of cell surface markers. Monocyte-derived dendritic cells (Mo-DCs) were cultured with dead endometrial stromal cells (dESCs) to investigate changes in phagocytic activity and cytokine expression.
MAIN OUTCOME MEASURE(S): Cell surface markers and cytokine expression and identification with the use of flow cytometry or reverse-transcription polymerase chain reaction (RT-PCR). Changes in cytokine expression and phagocytic activity of Mo-DCs cultured with dESCs and d-mannan were measured with the use of flow cytometry and RT-PCR.
RESULT(S): The proportion of mannose receptor (MR)-positive myeloid DC type 1 was higher in endometriosis samples than in control samples. The blocking of MR reduced phagocytosis of dESCs by Mo-DCs. Mo-DCs cultured with dESCs expressed higher levels of interleukin (IL) 1β and IL-6 than control samples.
CONCLUSION(S): Peritoneal DCs in endometriosis tissue express high levels of MR, which promotes phagocytosis of dead endometrial cells and thereby contributes to the etiology of endometriosis.
描述子宫内膜异位症中的腹膜树突状细胞（DC）并阐明其在发病机制中的作用。
实验性。
大学医院。
63 名女性（35 名子宫内膜异位症患者和 28 名对照女性），她们接受了腹腔镜手术。
分析子宫内膜异位症和对照样本中的腹膜 DC 表面标志物的表达。用死亡的子宫内膜基质细胞（dESC）培养单核细胞衍生的树突状细胞（Mo-DC），以研究吞噬活性和细胞因子表达的变化。
用流式细胞术或逆转录聚合酶链反应（RT-PCR）检测细胞表面标志物和细胞因子表达。用流式细胞术和 RT-PCR 测量用 dESC 和 d-甘露聚糖培养的 Mo-DC 中细胞因子表达和吞噬活性的变化。
子宫内膜异位症样本中甘露糖受体（MR）阳性髓样 DC 1 型的比例高于对照样本。MR 阻断减少了 Mo-DC 对 dESC 的吞噬作用。与对照样本相比，用 dESC 培养的 Mo-DC 表达更高水平的白细胞介素（IL）1β和 IL-6。
子宫内膜异位症组织中的腹膜 DC 表达高水平的 MR，促进了对死亡的子宫内膜细胞的吞噬作用，从而有助于子宫内膜异位症的发病机制。
