Department of Paediatrics, Chang Gung Memorial Hospital, Keelung Branch, Keelung, Taiwan; Chang Gung University College of Medicine, Taoyuan, Taiwan; Molecular Infectious Disease Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Department of Paediatrics, Chang Gung Memorial Hospital, Keelung Branch, Keelung, Taiwan; Chang Gung University College of Medicine, Taoyuan, Taiwan.
Clin Microbiol Infect. 2017 Feb;23(2):121.e1-121.e7. doi: 10.1016/j.cmi.2016.10.020. Epub 2016 Oct 26.
The study aimed to determine the long-term Staphylococcus aureus colonization patterns and strain relatedness, and the association between maternal and infant colonization in infancy.
A birth cohort study was conducted from January 2012 to November 2014. Nasopharyngeal swabs for S. aureus detection were collected from infants at the age of 1, 2, 4, 6 and 12 months and from mothers when their children were 1-month-old.
In total, 254 samples were collected at each planned visit during the first 12-month study. The prevalence of S. aureus colonization decreased in the first year of life, ranging from 61.0% (155/254) at the age of 1 month to 12.2% (31/254) at 12 months. Persistent colonization, defined as a positive culture on four or five occasions, was detected in only 13.8% (35/254) of carriers. Most of the persistent carriers were colonized with methicillin-resistant S. aureus (MRSA) only, and among persistent MRSA carriers, 61.1% (11/18) had indistinguishable genotypes. Of the mothers with MRSA colonization, 77.1% (27/35) had infants who were concomitantly colonized at the age of 1 month; 70.4% (19/27) of the infant-mother paired isolates belonged to indistinguishable or related subtypes, which suggests that surrounding carriers, probably their mothers, may be the possible source for MRSA acquisition in early infancy.
Staphylococcus aureus colonization including MRSA was commonly observed in our cohort. Strains of persistent MRSA among infant-mother pairs were usually of indistinguishable genotypes. Therefore, horizontal spread within households is possibly an important factor related to infant MRSA colonization.
本研究旨在确定金黄色葡萄球菌（Staphylococcus aureus）的长期定植模式和菌株相关性，以及母婴在婴儿期定植的相关性。
本研究为一项从 2012 年 1 月至 2014 年 11 月开展的出生队列研究。在婴儿 1、2、4、6 和 12 个月龄时，以及母亲在其孩子 1 个月龄时，采集鼻咽拭子以检测金黄色葡萄球菌。
在第 1 年的 12 次计划访视中，共采集了 254 份样本。金黄色葡萄球菌定植率在生命的第一年逐渐下降，1 月龄时为 61.0%（155/254），12 月龄时为 12.2%（31/254）。仅 13.8%（35/254）的定植者出现持续定植（4 或 5 次培养阳性）。大多数持续定植者仅定植耐甲氧西林金黄色葡萄球菌（MRSA），在持续定植的 MRSA 携带者中，61.1%（11/18）的基因型相同。MRSA 定植的母亲中，77.1%（27/35）的婴儿在 1 月龄时同时定植；70.4%（19/27）的母婴配对分离株属于相同或相关的亚型，这表明周围的定植者，可能是他们的母亲，可能是婴儿早期 MRSA 定植的可能来源。
金黄色葡萄球菌定植（包括 MRSA）在本队列中很常见。母婴配对的持续 MRSA 菌株通常具有相同的基因型。因此，家庭内的水平传播可能是与婴儿 MRSA 定植相关的一个重要因素。
