Jolly J, Goldberg M
Med Lab Sci. 1989 Jan;46(1):2-5.
Ninety-four strains of coagulase positive staphylococci and 73 strains of coagulase negative staphylococci were tested for their susceptibility to methicillin, using agar dilution and disc diffusion techniques with Diagnostic Sensitivity Test agar (DST) and DST supplemented with salt, incubated at 30 degrees C and 37 degrees C. Disc diffusion on salt-incorporated DST at 30 degrees C was most reliable for detecting methicillin resistance in both types of staphylococci. The agar dilution methods were particularly poor for detecting resistance with coagulase negative staphylococci. The simultaneous testing of coagulase positive and negative staphylococci by this method indicated the need for both DST and salt-incorporated DST agar plates, incubated at 30 degrees C. However, sensitive strains should still be tested by disc diffusion for confirmation.
采用琼脂稀释法和纸片扩散法，使用诊断敏感性试验琼脂（DST）以及添加盐的DST，在30℃和37℃下孵育，对94株凝固酶阳性葡萄球菌和73株凝固酶阴性葡萄球菌进行了甲氧西林敏感性测试。在30℃下，在添加盐的DST上进行纸片扩散法对于检测两种葡萄球菌中的甲氧西林耐药性最为可靠。琼脂稀释法在检测凝固酶阴性葡萄球菌的耐药性方面尤其不佳。通过该方法同时检测凝固酶阳性和阴性葡萄球菌表明，需要30℃孵育的DST和添加盐的DST琼脂平板。然而，敏感菌株仍应通过纸片扩散法进行检测以进行确认。
