Diller D L, Flournoy D J
Department of Clinical Laboratory Sciences, College of Allied Health, University of Oklahoma Health Sciences Center, Oklahoma City.
Methods Find Exp Clin Pharmacol. 1988 Nov;10(11):695-7.
Ninety two consecutively occurring clinical isolates of Staphylococcus aureus and 71 coagulase negative staphylococci (CONS) were tested by disc agar diffusion (DAD) and agar dilution (Mueller Hinton agar with 4% sodium chloride and 6 mcg/ml oxacillin) for methicillin resistance. There were 6 S. aureus and 33 CONS which were resistant to oxacillin. Five discrepancies (CONS) occurred between the two methods, with all indicating susceptibility by DAD and resistance by agar dilution. Four discrepancies were resolved by minimal inhibitory concentration (MIC) tests. In two of the CONS, the MIC agreed with the DAD results, and in the other two, the MIC agreed with the agar dilution results. Results of this study indicate that DAD is an accurate and acceptable method of detecting methicillin resistance in staphylococci (especially S. aureus) at this institution.
采用纸片琼脂扩散法（DAD）和琼脂稀释法（含4%氯化钠和6微克/毫升苯唑西林的穆勒-欣顿琼脂）对92株连续分离的金黄色葡萄球菌临床分离株和71株凝固酶阴性葡萄球菌（CONS）进行耐甲氧西林检测。有6株金黄色葡萄球菌和33株CONS对苯唑西林耐药。两种方法之间出现了5个差异（CONS），所有差异均表明DAD法检测为敏感，而琼脂稀释法检测为耐药。通过最低抑菌浓度（MIC）试验解决了4个差异。在2株CONS中，MIC结果与DAD法一致，在另外2株中，MIC结果与琼脂稀释法一致。本研究结果表明，在该机构，DAD法是检测葡萄球菌（尤其是金黄色葡萄球菌）耐甲氧西林的一种准确且可接受的方法。
