Dai Xurui, Thongchot Suyanee, Dokduang Hasaya, Loilome Watcharin, Khuntikeo Narong, Titapun Attapol, Ungarreevittaya Piti, Yongvanit Puangrat, Techasen Anchalee, Namwat Nisana
Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand.
Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen, Thailand.
Anticancer Res. 2016 Nov;36(11):5981-5988. doi: 10.21873/anticanres.11186.
We examined the in vitro effects of the selenium compounds sodium selenite (Se) and selenomethionine (SeMet) on cholangiocarcima (CCA) cell growth and migration to determine their potential usefulness as anticancer agents. The effect of both compounds on the selenoprotein M level was investigated, as well as the association between the expression level of selenoprotein M and the patients' clinicopathological data. Se and SeMet inhibited CCA cell growth with half-maximal inhibitory concentration values of 1.7-2.1 μM and 18.8-37.9 μM, respectively. Both compounds increased the ratio of B-cell lymphoma 2 (BCL2) to BCL2-associated X (BAX), triggering apoptotic cell death, and inhibited cell migration by reducing the ratio of N-cadherin to E-cadherin, an epithelial-mesenchymal transition marker. In addition, Se and SeMet increased selenoprotein M protein in CCA cells. Low expression of selenoprotein M in CCA tissues was significantly associated with shorter patient survival. In conclusion, selenium may potentially be an alternative anticancer agent that might lead to a better prognosis in patients with CCA.
我们研究了亚硒酸钠（Se）和硒代蛋氨酸（SeMet）这两种硒化合物对胆管癌（CCA）细胞生长和迁移的体外影响，以确定它们作为抗癌剂的潜在效用。研究了这两种化合物对硒蛋白M水平的影响，以及硒蛋白M表达水平与患者临床病理数据之间的关联。Se和SeMet抑制CCA细胞生长，其半数最大抑制浓度值分别为1.7 - 2.1 μM和18.8 - 37.9 μM。两种化合物均增加了B细胞淋巴瘤2（BCL2）与BCL2相关X蛋白（BAX）的比例，引发凋亡性细胞死亡，并通过降低上皮-间质转化标志物N-钙黏蛋白与E-钙黏蛋白的比例来抑制细胞迁移。此外，Se和SeMet增加了CCA细胞中硒蛋白M的含量。CCA组织中硒蛋白M的低表达与患者较短的生存期显著相关。总之，硒可能是一种潜在的抗癌剂，有望改善CCA患者的预后。
