Landini M P, Varoli O, Tucci A, Paparo G F, Corinaldesi R, Zoccoli G, Lalli A A, La Placa M
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Università di Bologna, Italy.
Microbiologica. 1989 Jul;12(3):181-8.
We used Western Blotting analysis to determine the immune profile to Campylobacter pylori polypeptides in: A) sera from patients with idiopathic dyspepsia and bacteriological evidence of C. pylori gastric colonization, B) sera from patients with the same symptoms but no bacteriological evidence of C. pylori infection and C) healthy subjects. To avoid interference of aspecific reactions due to antigenic cross reactivity with other thermophilic Campylobacter species, antisera were raised in rabbits against C. pylori as well as against C. coli and C. jejuni. Some bands (with an approximate molecular weight of 118, 85, 40, 34, 28, 18 and 12 Kd) which can be considered specific for C. pylori were identified and the IgG reaction to some of them (40, 34, 28 Kd) was shown to be significantly higher in patients with bacteriological evidence of C. pylori infection than in the other two groups. IgM reactivity to two bacterial proteins of molecular weight 118 and 40 Kd was particularly evident in the second group of patients suggesting a possible diagnostic tool to identify C. pylori infection at a very early stage.
我们采用蛋白质印迹分析来确定针对幽门螺杆菌多肽的免疫谱，研究对象包括：A）患有特发性消化不良且有幽门螺杆菌胃定植细菌学证据的患者的血清；B）有相同症状但无幽门螺杆菌感染细菌学证据的患者的血清；C）健康受试者。为避免因与其他嗜热弯曲菌属发生抗原交叉反应而产生非特异性反应的干扰，用幽门螺杆菌、大肠弯曲菌和空肠弯曲菌免疫家兔制备抗血清。鉴定出了一些可认为是幽门螺杆菌特异性的条带（分子量约为118、85、40、34、28、18和12千道尔顿），并且有细菌学证据表明感染幽门螺杆菌的患者对其中一些条带（40、34、28千道尔顿）的IgG反应显著高于其他两组。在第二组患者中，对分子量为118和40千道尔顿的两种细菌蛋白的IgM反应尤为明显，这表明可能是一种在极早期识别幽门螺杆菌感染的诊断工具。
