Golde D W, Bersch N, Li C H
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3437-9. doi: 10.1073/pnas.75.7.3437.
There are few studies showing a biological effect of growth hormone (somatotropin) on cell proliferation in vitro at physiological concentrations. We report here that Friend virus-infected erythroleukemia cells are responsive to growth hormone in vitro. Using a serum-free clonogenic assay we found as little as 0.1 ng of human growth hormone per ml caused a prominent stimulation of cell proliferation. Peak activity of human growth hormone occurred at 200 ng/ml, resulting in a 2-fold increase in cloning. Human chorionic somatomammotropin and the Cys(Cam)53-hGH(1-134) fragment of human growth hormone were also active, but a biologically inert oxidized human growth hormone had no growth-promoting effect in vitro. Cell proliferation was stimulated by insulin with peak potentiation occurring at 1 ng/ml, and prolactin had a demonstrable stimulatory effect between 50 and 100 ng/ml. These observations indicate that growth hormone and related polypeptides have a direct effect on the in vitro proliferation of erythroleukemia cells in the absence of serum. The results confirm a direct action of growth hormone on mammalian cells and suggest that pituitary hormones may affect leukemic cell growth.
很少有研究表明生长激素（促生长素）在生理浓度下对体外细胞增殖具有生物学效应。我们在此报告，感染了弗氏病毒的红白血病细胞在体外对生长激素有反应。使用无血清克隆形成试验，我们发现每毫升低至0.1纳克的人生长激素就能显著刺激细胞增殖。人生长激素的峰值活性出现在200纳克/毫升时，导致克隆增加了两倍。人绒毛膜促生长催乳素和人生长激素的Cys(Cam)53-hGH(1-134)片段也具有活性，但一种无生物学活性的氧化型人生长激素在体外没有促生长作用。胰岛素可刺激细胞增殖，峰值增强出现在1纳克/毫升时，催乳素在50至100纳克/毫升之间有明显的刺激作用。这些观察结果表明，在无血清的情况下，生长激素和相关多肽对红白血病细胞的体外增殖有直接影响。结果证实了生长激素对哺乳动物细胞的直接作用，并表明垂体激素可能影响白血病细胞的生长。
