Myatt D C, Davis G H
Department of Microbiology, University of Queensland, St. Lucia, Australia.
Med Microbiol Immunol. 1989;178(5):279-87. doi: 10.1007/BF00191062.
Twenty-seven Vibrio strains were assessed for virulence-associated biological activities, including iron chelation, hydrolases, haemolysis and haemagglutination. All strains hydrolysed DNA, chitin, gelatin and casein, produced siderophores, and lysed red blood cells. All V. fluvialis, V. cholerae and V. mimicus strains exhibited diverse lipolytic activity distinct from more discriminate lipolysis by V. furnissii. V. furnissii manifested fibrin and mucin hydrolysis but no phosphate or esculin hydrolysis, for which V. fluvialis varied. No strains hydrolysed urea, alginate or keratin. V. fluvialis, V. furnissii and V. mimicus strains failed to exhibit the mannose-sensitive haemagglutination typical of V. cholerae. Some activities may distinguish otherwise phenotypically similar species. Species tested commonly possessed biological activities that may contribute to virulence, although there was no apparent correlation with isolation from human sources.
对27株弧菌菌株进行了与毒力相关的生物学活性评估，包括铁螯合、水解酶、溶血和血凝。所有菌株均能水解DNA、几丁质、明胶和酪蛋白，产生铁载体，并裂解红细胞。所有河流弧菌、霍乱弧菌和拟态弧菌菌株均表现出不同的脂解活性，这与弗氏弧菌更具特异性的脂解作用不同。弗氏弧菌表现出纤维蛋白和粘蛋白水解，但不表现出磷酸盐或七叶苷水解，而河流弧菌在这方面存在差异。没有菌株能水解尿素、藻酸盐或角蛋白。河流弧菌、弗氏弧菌和拟态弧菌菌株未表现出霍乱弧菌典型的甘露糖敏感血凝。某些活性可能会区分其他表型相似的菌种。所测试的菌种通常具有可能有助于毒力的生物学活性，尽管与从人类来源分离没有明显的相关性。
