Nishibuchi M, Seidler R J
Appl Environ Microbiol. 1983 Jan;45(1):228-31. doi: 10.1128/aem.45.1.228-231.1983.
Fluid accumulation at 4 h in the intestines of suckling mice enabled us to distinguish non-O-1 Vibrio cholerae, V. mimicus, and V. fluvialis clinical isolates from environmental isolates. Enterotoxin production was culture medium dependent. Filtrates of cultures grown in tryptic soy broth without glucose but with added 0.5% NaCl did not exhibit marked enterotoxin activity in the assay. Culture filtrates of all clinical strains grown in brain heart infusion broth supplemented with 0.5% NaCl induced large amounts of fluid accumulation in mouse intestines. However, most environmental strains grown in brain heart infusion broth amended as described above were unable to induce fluid accumulation. The enterotoxin present in culture filtrates lost activity at 56 degrees C and appeared to be distinct from previously described virulence factors, including the well-described cholera toxin. The new enterotoxin could represent an important virulence mechanism common to all three species.
哺乳小鼠肠道在4小时时的液体蓄积使我们能够区分非O-1群霍乱弧菌、拟态弧菌和河流弧菌的临床分离株与环境分离株。肠毒素的产生依赖于培养基。在不含葡萄糖但添加了0.5%氯化钠的胰蛋白胨大豆肉汤中培养的滤液在检测中未表现出明显的肠毒素活性。在补充了0.5%氯化钠的脑心浸液肉汤中培养的所有临床菌株的培养滤液在小鼠肠道中诱导了大量液体蓄积。然而，在上述改良的脑心浸液肉汤中培养的大多数环境菌株无法诱导液体蓄积。培养滤液中存在的肠毒素在56℃时失去活性，并且似乎与先前描述的毒力因子不同，包括广为人知的霍乱毒素。这种新的肠毒素可能代表了这三个物种共有的一种重要毒力机制。
