Chin A C, Fournier R E
Department of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Mol Cell Biol. 1989 Sep;9(9):3736-43. doi: 10.1128/mcb.9.9.3736-3743.1989.
Serum albumin gene expression is generally extinguished in hepatoma x fibroblast hybrids. To define the genetic basis of this phenomenon, we screened a panel of hepatoma hybrids retaining different fibroblast chromosomes for albumin production by immunofluorescence. We report that albumin extinction in these clones was strictly correlated with the retention of mouse chromosome 1. Furthermore, albumin was systematically reexpressed in chromosome 1 segregants. These data define a tissue-specific extinguisher locus (Tse-2) that affects albumin gene expression in trans. Two other liver genes, those encoding liver alcohol dehydrogenase and liv-10, were coordinately extinguished with albumin in monochromosomal hybrids that specifically retained mouse chromosome 1.
血清白蛋白基因表达在肝癌细胞与成纤维细胞的杂交细胞中通常会被抑制。为了确定这种现象的遗传基础，我们通过免疫荧光筛选了一组保留不同成纤维细胞染色体的肝癌杂交细胞系，以检测白蛋白的产生情况。我们报告称，这些克隆中白蛋白的抑制与小鼠1号染色体的保留严格相关。此外，在1号染色体分离株中白蛋白系统性地重新表达。这些数据定义了一个反式作用于白蛋白基因表达的组织特异性抑制基因座（Tse-2）。在特异性保留小鼠1号染色体的单染色体杂交细胞中，另外两个肝脏基因，即编码肝脏乙醇脱氢酶和liv-10的基因，与白蛋白协同被抑制。
