Botticella Ermelinda, Sestili Francesco, Ferrazzano Gianluca, Mantovani Paola, Cammerata Alessandro, D'Egidio Maria Grazia, Lafiandra Domenico
Department of Agricultural and Forestry Sciences, University of Tuscia , Via San Camillo De Lellis, 01100 Viterbo , Italy.
Società Produttori Sementi , Via Macero 1; 40050 Argelato (BO) , Italy.
Breed Sci. 2016 Sep;66(4):572-579. doi: 10.1270/jsbbs.16025. Epub 2016 Jul 21.
Starch represents a major nutrient in the human diet providing essentially a source of energy. More recently the modification of its composition has been associated with new functionalities both at the nutritional and technological level. Targeting the major starch biosynthetic enzymes has been shown to be a valuable strategy to manipulate the amylose-amylopectin ratio in reserve starch. In the present work a breeding strategy aiming to produce a set of SSIIa (starch synthases IIa) durum wheat is described. We have characterized major traits such as seed weight, total starch, amylose, protein and β-glucan content in a set of mutant families derived from the introgression of the SSIIa trait into Svevo, an elite Italian durum wheat cultivar. A large degree of variability was detected and used to select wheat lines with either improved quality traits or agronomic performances. Semolina of a set of two SSIIa lines showed new rheological behavior and an increased content of all major dietary fiber components, namely arabinoxylans, β-glucans and resistant starch. Furthermore the investigation of gene expression highlighted important differences in some genes involved in starch and β-glucans biosynthesis.
淀粉是人类饮食中的主要营养成分，主要提供能量来源。最近，其成分的改变在营养和技术层面都与新的功能相关联。针对主要的淀粉生物合成酶已被证明是一种操纵储备淀粉中直链淀粉与支链淀粉比例的有效策略。在本研究中，描述了一种旨在培育一组含有淀粉合成酶IIa（SSIIa）的硬粒小麦的育种策略。我们对一组源自将SSIIa性状导入意大利优质硬粒小麦品种斯韦沃（Svevo）的突变家系中的主要性状进行了表征，如种子重量、总淀粉、直链淀粉、蛋白质和β-葡聚糖含量。检测到了很大程度的变异性，并用于选择具有改良品质性状或农艺性能的小麦品系。一组两个含有SSIIa的品系的粗粒粉表现出了新的流变学行为，并且所有主要膳食纤维成分（即阿拉伯木聚糖、β-葡聚糖和抗性淀粉）的含量都有所增加。此外，基因表达研究突出了一些参与淀粉和β-葡聚糖生物合成的基因的重要差异。
