Tang Xilan, Gan Xiaohong Tracey, Rajapurohitam Venkatesh, Huang Cathy Xiaoling, Xue Jenny, Lui Edmund M K, Karmazyn Morris
Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON N6A 5C1, Canada.
Can J Physiol Pharmacol. 2016 Dec;94(12):1325-1335. doi: 10.1139/cjpp-2016-0337. Epub 2016 Aug 17.
There is increasing evidence for a beneficial effect of ginseng on cardiac pathology. Here, we determined whether North American ginseng can modulate the deleterious effects of the β-adrenoceptor agonist isoproterenol on cardiac hypertrophy and function using in vitro and in vivo approaches. Isoproterenol was administered for 2 weeks at either 25 mg/kg per day or 50 mg/kg per day (ISO25 or ISO50) via a subcutaneously implanted osmotic mini-pump to either control rats or those receiving ginseng (0.9 g/L in the drinking water ad libitum). Isoproterenol produced time- and dose-dependent left ventricular dysfunction, although these effects were attenuated by ginseng. Improved cardiac functions were associated with reduced heart masses, as well as prevention in the upregulation of the hypertrophy-related fetal gene expression. Lung masses were similarly attenuated, suggesting reduced pulmonary congestion. In in vitro studies, ginseng (10 μg/mL) completely suppressed the hypertrophic response to 1 μmol/L isoproterenol in terms of myocyte surface area, as well as reduction in the upregulation of fetal gene expression. These effects were associated with attenuation in both protein kinase A and cAMP response element-binding protein phosphorylation. Ginseng attenuates adverse cardiac adrenergic responses and, therefore, may be an effective therapy to reduce hypertrophy and heart failure associated with excessive catecholamine production.
越来越多的证据表明人参对心脏病理具有有益作用。在此，我们使用体外和体内方法确定北美人参是否可以调节β-肾上腺素能受体激动剂异丙肾上腺素对心脏肥大和功能的有害影响。通过皮下植入的渗透微型泵，以每天25mg/kg或50mg/kg（ISO25或ISO50）的剂量给对照大鼠或接受人参（饮用水中0.9g/L，随意饮用）的大鼠连续注射异丙肾上腺素2周。异丙肾上腺素产生了时间和剂量依赖性的左心室功能障碍，尽管这些影响被人参减弱。心脏功能的改善与心脏重量减轻以及肥大相关胎儿基因表达上调的预防有关。肺重量也同样减轻，表明肺充血减少。在体外研究中，就心肌细胞表面积而言，人参（10μg/mL）完全抑制了对1μmol/L异丙肾上腺素的肥大反应，以及胎儿基因表达上调的减少。这些影响与蛋白激酶A和环磷酸腺苷反应元件结合蛋白磷酸化的减弱有关。人参减弱了不良的心脏肾上腺素能反应，因此，可能是减少与儿茶酚胺过度产生相关的肥大和心力衰竭的有效疗法。
