Bajar Bryce T, Lam Amy J, Badiee Ryan K, Oh Young-Hee, Chu Jun, Zhou Xin X, Kim Namdoo, Kim Benjamin B, Chung Mingyu, Yablonovitch Arielle L, Cruz Barney F, Kulalert Kanokwan, Tao Jacqueline J, Meyer Tobias, Su Xiao-Dong, Lin Michael Z
Department of Bioengineering, Stanford University, Stanford, California, USA.
Department of Pediatrics, Stanford University, Stanford, California, USA.
Nat Methods. 2016 Dec;13(12):993-996. doi: 10.1038/nmeth.4045. Epub 2016 Oct 31.
A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.
非常需要一种能同时可视化活细胞中所有四个细胞周期阶段的强大方法。我们开发了一种从S期到G2期转变的强度测定报告基因,并设计了一种远红荧光蛋白mMaroon1,以可视化有丝分裂中的染色质凝聚。我们将这些新的报告基因与先前描述的Fucci系统相结合,创建了Fucci4,这是一组四个正交荧光指示剂,共同解析所有细胞周期阶段。