使用μManager软件的高级显微镜控制方法。
Advanced methods of microscope control using μManager software.
作者信息
Edelstein Arthur D, Tsuchida Mark A, Amodaj Nenad, Pinkard Henry, Vale Ronald D, Stuurman Nico
机构信息
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
Luminous Point, LLC, 626 Prentiss St, San Francisco, CA 94110, USA.
出版信息
J Biol Methods. 2014;1(2). doi: 10.14440/jbm.2014.36.
μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.
μManager是一款开源的跨平台桌面应用程序，用于控制各种电动显微镜、科学相机、载物台、照明器及其他显微镜附件。自2005年创建以来，μManager已发展到支持多种显微镜硬件，目前全球有成千上万的研究人员使用它。该应用程序提供了成熟的图形用户界面，并提供开放的编程接口以方便插件和脚本的使用。在此，我们介绍一些最近添加的μManager高级功能的使用指南，包括硬件同步、多台相机同时使用、将图案光投射到样本上、实时载玻片映射、多孔板成像、粒子定位与跟踪以及高速成像。
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