伊朗西北部菌株的分子多样性
Molecular Diversity of Strains in Northwestern Iran.
作者信息
Pourostadi Mahya, Rashedi Jalil, Mahdavi Poor Behroz, Samadi Kafil Hossein, Shirazi Samaneh, Asgharzadeh Mohammad
机构信息
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran.
Tuberculosis and Lung Disease Research Center, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, IR Iran.
出版信息
Jundishapur J Microbiol. 2016 Aug 31;9(9):e35520. doi: 10.5812/jjm.35520. eCollection 2016 Sep.
BACKGROUND
Years after the development of antituberculosis (TB) drugs, many people continue to suffer from this disease. To control the spread of TB, strains of the complex need to be determined, and sources of infection must be identified. Such steps should help to prevent transmission of the infection.
OBJECTIVES
The aim of this study was to perform molecular genotyping of isolates of the complex obtained from patients in northwestern Iran.
METHODS
One hundred ninety-four culture-positive isolates obtained from patients in northwestern Iran were analyzed using the mycobacterial interspersed repetitive unit-exact tandem repeats (MIRU-ETR) method.
RESULTS
The MIRU-ETR method distinguished 162 different patterns in the 194 isolates, comprising 23 clusters and 139 unique patterns. Its discriminatory power according to the Hunter-Gaston discriminatory index (HGDI) was 0.9978. The largest cluster contained six isolates.
CONCLUSIONS
This research indicated that various strains of were responsible for TB and that the majority of cases were due to reactivation.
背景
在抗结核药物研发多年后，仍有许多人罹患此病。为控制结核病传播，需确定结核分枝杆菌复合群菌株，并找出感染源。此类措施应有助于预防感染传播。
目的
本研究旨在对从伊朗西北部患者分离出的结核分枝杆菌复合群菌株进行分子基因分型。
方法
采用分枝杆菌间散布重复单位 - 精确串联重复序列（MIRU - ETR）方法，对从伊朗西北部患者分离出的194株培养阳性的结核分枝杆菌复合群菌株进行分析。
结果
MIRU - ETR方法在194株菌株中鉴别出162种不同模式，包括23个簇和139种独特模式。根据亨特 - 加斯顿鉴别指数（HGDI），其鉴别能力为0.9978。最大的簇包含6株菌株。
结论
本研究表明，多种结核分枝杆菌复合群菌株可导致结核病，且大多数病例是由复发引起的。
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